C57BL/6JCya-Atp1b1em1/Cya
Common Name:
Atp1b1-KO
Product ID:
S-KO-18712
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Atp1b1-KO
Strain ID
KOCMP-11931-Atp1b1-B6J-VB
Gene Name
Product ID
S-KO-18712
Gene Alias
Atp4b; Atpb; Atpb-1; NKbeta1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Atp1b1em1/Cya mice (Catalog S-KO-18712) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027863
NCBI RefSeq
NM_009721
Target Region
Exon 3~4
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Atp1b1, encoding the Na,K-ATPase β subunit, is a key regulator of the Na+ and K+ electrochemical gradients across the plasma membrane, essential for cellular activity. It is involved in diverse biological processes such as maintaining cellular homeostasis, membrane potential, and is associated with pathways like focal adhesion [2,3,4].
In antiviral innate immunity, increased expression of Atp1b1 after DNA and RNA virus infections can inhibit viral replication and up-regulate the levels of IFNs, IFN-stimulated genes, and inflammatory cytokines. It does so by interacting with TRAF3 and TRAF6, potentiating their ubiquitination and leading to increased phosphorylation of downstream molecules [1].
In diffuse large B-cell lymphoma (DLBCL), Atp1b1 is overexpressed in DLBCL cell lines compared to CD19 + B cells. Down-regulation of Atp1b1 inhibits DLBCL cell proliferation, migration, invasion, and adhesion, suggesting it could be a diagnostic biomarker and therapeutic target [2].
In cytogenetically normal acute myeloid leukemia (CN-AML), high Atp1b1 expression is associated with shorter overall survival and event-free survival, and up-regulation of certain oncogenes is associated with high Atp1b1 expression, indicating its leukemogenicity [3].
In conclusion, Atp1b1 plays crucial roles in maintaining cellular ion gradients and normal cellular functions. Studies have revealed its significance in antiviral responses, and its potential as a biomarker and therapeutic target in diseases like DLBCL and CN-AML. Research on Atp1b1 using various models helps to better understand its functions in different biological processes and disease conditions.
References：
1. Cao, Wei, Guo, Yifei, Cheng, Zhikui, Liu, Shi, Zhu, Ying. 2021. Inducible ATP1B1 Upregulates Antiviral Innate Immune Responses by the Ubiquitination of TRAF3 and TRAF6. In Journal of immunology (Baltimore, Md. : 1950), 206, 2668-2681. doi:10.4049/jimmunol.2001262. https://pubmed.ncbi.nlm.nih.gov/34011520/
2. Zhang, Shuo, Wang, Hongmin, Liu, Aichun. . Identification of ATP1B1, a key copy number driver gene in diffuse large B-cell lymphoma and potential target for drugs. In Annals of translational medicine, 10, 1136. doi:10.21037/atm-22-4709. https://pubmed.ncbi.nlm.nih.gov/36388804/
3. Shi, Jin-long, Fu, Lin, Ang, Qing, Zhu, Jun, Wang, Wei-dong. . Overexpression of ATP1B1 predicts an adverse prognosis in cytogenetically normal acute myeloid leukemia. In Oncotarget, 7, 2585-95. doi:10.18632/oncotarget.6226. https://pubmed.ncbi.nlm.nih.gov/26506237/
4. Schneider, Constanze, Spaink, Hermes, Alexe, Gabriela, Oellerich, Thomas, Stegmaier, Kimberly. . Targeting the Sodium-Potassium Pump as a Therapeutic Strategy in Acute Myeloid Leukemia. In Cancer research, 84, 3354-3370. doi:10.1158/0008-5472.CAN-23-3560. https://pubmed.ncbi.nlm.nih.gov/39024560/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen