C57BL/6NCya-Ppiaem1/Cya
Common Name:
Ppia-KO
Product ID:
S-KO-18715
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Ppia-KO
Strain ID
KOCMP-268373-Ppia-B6N-VA
Gene Name
Product ID
S-KO-18715
Gene Alias
Cphn; CyP-18; CypA; SP18
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ppiaem1/Cya mice (Catalog S-KO-18715) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000132846
NCBI RefSeq
NM_008907
Target Region
Exon 1~5
Size of Effective Region
~7.3 kb
Detailed Document
Overview of Gene Research
Ppia, also known as peptidylprolyl isomerase A or cyclophilin A, is a central enzyme in the protein-folding response pathway [3]. It has peptidyl-prolyl cis/trans isomerase activity, which can catalyze the cis-trans isomerization of peptidyl-prolyl bonds in proteins, thus playing a crucial role in protein folding, assembly, and transport. Ppia is involved in multiple biological pathways and is important for normal cellular function [5].
In lung cancer, ablation of Ppia promotes NRF2 protein degradation and blocks NRF2-driven growth, indicating its role in promoting lung cancer progression [1]. Ppia -/- mice have significantly less fat and smaller adipocytes, and silencing Ppia in cell lines impairs adipocyte differentiation, suggesting it is a novel adipogenic factor in obesity [2]. In multiple myeloma, CRISPR-Cas9 deletion of PPIA or its inhibition with a small molecule inhibitor sensitizes tumor cells to proteasome inhibitors, highlighting it as a potential therapeutic target [3]. In oral cancer, reducing PPIA expression and secretion decreases cell invasion and migration, while recombinant PPIA treatment increases these processes, suggesting it may be a novel target and prognostic marker [4].
In conclusion, Ppia is essential for protein-folding-related functions and is involved in various disease-related biological processes. Studies using gene-knockout models, such as Ppia -/- mice, have revealed its role in cancer progression, obesity, and multiple myeloma, providing valuable insights into potential therapeutic strategies for these diseases.
References:
1. Lu, Weiqiang, Cui, Jiayan, Wang, Wanyan, Ji, Hongbin, Huang, Jin. 2024. PPIA dictates NRF2 stability to promote lung cancer progression. In Nature communications, 15, 4703. doi:10.1038/s41467-024-48364-4. https://pubmed.ncbi.nlm.nih.gov/38830868/
2. Zhang, Lihong, Li, Zhen, Zhang, Bo, He, Haiyang, Bai, Yun. 2015. PPIA is a novel adipogenic factor implicated in obesity. In Obesity (Silver Spring, Md.), 23, 2093-100. doi:10.1002/oby.21208. https://pubmed.ncbi.nlm.nih.gov/26347493/
3. Cohen, Yael C, Zada, Mor, Wang, Shuang-Yin, Weiner, Assaf, Amit, Ido. 2021. Identification of resistance pathways and therapeutic targets in relapsed multiple myeloma patients through single-cell sequencing. In Nature medicine, 27, 491-503. doi:10.1038/s41591-021-01232-w. https://pubmed.ncbi.nlm.nih.gov/33619369/
4. Liao, En-Chi, Law, Ching-Hsuan, Chen, Hsin-Yi, Chou, Hsiu-Chuan, Chan, Hong-Lin. 2025. PPIA enhances cell growth and metastasis through CD147 in oral cancer. In Archives of biochemistry and biophysics, 765, 110328. doi:10.1016/j.abb.2025.110328. https://pubmed.ncbi.nlm.nih.gov/39921142/
5. Trémillon, Nicolas, Morello, Eric, Llull, Daniel, Ginisty, Hervé, Poquet, Isabelle. 2012. PpiA, a surface PPIase of the cyclophilin family in Lactococcus lactis. In PloS one, 7, e33516. doi:10.1371/journal.pone.0033516. https://pubmed.ncbi.nlm.nih.gov/22442694/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen