C57BL/6JCya-Rangap1em1/Cya
Common Name:
Rangap1-KO
Product ID:
S-KO-18723
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rangap1-KO
Strain ID
KOCMP-19387-Rangap1-B6J-VA
Gene Name
Product ID
S-KO-18723
Gene Alias
Fug1; mKIAA1835
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rangap1em1/Cya mice (Catalog S-KO-18723) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000052374
NCBI RefSeq
NM_001146174
Target Region
Exon 3~4
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
RanGAP1, short for Ran GTPase-activating protein 1, is crucial for maintaining mitotic chromosome integrity. It anchors to the kinetochore during mitosis, recruits PP1-γ to counteract the spindle-assembly checkpoint (SAC) activity, and prevents TOP2A degradation, safeguarding chromatid decatenation. It is also involved in nuclear protein export, bone development, and various signaling pathways [1,3,2].
In rapidly expanding mouse osteoprogenitors, RanGAP1 deficiency causes chromothripsis in chr1q, instant inactivation of Rb1, degradation of p53, and failure in DNA damage repair, leading to ultrafast osteosarcoma tumorigenesis [1]. In limb and head mesenchymal progenitor cells expressing Prx1 in mice, ablation of RanGAP1 results in embryonic lethality, severe cartilage and bone dysplasia, and triggers missegregation of chromosomes, inhibiting the expression of key genes in bone development signaling pathways [2].
In conclusion, RanGAP1 is essential for maintaining chromosome stability during cell division and in bone development. The RanGAP1 knockout mouse models have revealed its role in promoting osteosarcoma tumorigenesis and its importance in skeletal development. Understanding RanGAP1 can provide insights into the mechanisms of tumorigenesis and bone-related diseases.
References：
1. Gong, Yan, Zou, Shitian, Deng, Daizhao, Zou, Zhipeng, Bai, Xiaochun. 2023. Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma. In Developmental cell, 58, 192-210.e11. doi:10.1016/j.devcel.2022.12.012. https://pubmed.ncbi.nlm.nih.gov/36696903/
2. Huang, Minjun, Chen, Bochong, Chen, Xiaoli, Bai, Xiaochun, Gong, Yan. 2024. RanGAP1 maintains chromosome stability in limb bud mesenchymal cells during bone development. In Cellular signalling, 120, 111222. doi:10.1016/j.cellsig.2024.111222. https://pubmed.ncbi.nlm.nih.gov/38729327/
3. Tripathi, Brajendra K, Hirsh, Nicole H, Qian, Xiaolan, Walters, Kylie J, Lowy, Douglas R. 2024. The pro-oncogenic noncanonical activity of a RAS•GTP:RanGAP1 complex facilitates nuclear protein export. In Nature cancer, 5, 1902-1918. doi:10.1038/s43018-024-00847-5. https://pubmed.ncbi.nlm.nih.gov/39528835/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen