C57BL/6JCya-Ptges2em1/Cya
Common Name:
Ptges2-KO
Product ID:
S-KO-18725
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ptges2-KO
Strain ID
KOCMP-96979-Ptges2-B6J-VA
Gene Name
Product ID
S-KO-18725
Gene Alias
0610038H10Rik; Gbf1; Mpges2; Pges2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ptges2em1/Cya mice (Catalog S-KO-18725) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028162
NCBI RefSeq
NM_133783
Target Region
Exon 3~7
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Ptges2, also known as microsomal prostaglandin E synthase-2 (mPGES-2), is an enzyme involved in the synthesis of prostaglandin E2 (PGE2) from COX-derived PGH2 [4]. It is part of the arachidonic acid metabolism pathway and is of great biological importance in regulating various physiological and pathological processes [5]. Genetic models, such as KO/CKO mouse models, are valuable tools for studying its functions.
In AKI models, both global and tubule-specific mPGES-2-deficient mice treated with cisplatin or subjected to unilateral renal ischemia/reperfusion showed decreased renal dysfunction and morphological damage, indicating that mPGES-2 blockage may be a promising therapeutic strategy, likely through inhibiting ferroptosis via the heme-dependent regulation of the p53/SLC7A11/GPX4 axis [2]. In DKD models, global knockout or pharmacological blockage of mPGES-2 attenuated diabetic podocyte injury and tubulointerstitial fibrosis, ameliorating lipid accumulation and lipotoxicity, with mPGES-2 and Rev-Erbα competing for heme binding to regulate fatty acid binding protein 5 expression and lipid metabolism in the diabetic kidney [3]. In the context of brown fat, METTL14-mediated m6A promotes the decay of Ptges2, and BAT-specific knockdown of Ptges2 reverses the insulin-sensitizing effects in M14KO mice, revealing a role of Ptges2 in regulating systemic insulin sensitivity [1].
In conclusion, Ptges2 is crucial in the synthesis of PGE2 and participates in multiple biological processes. Studies using KO/CKO mouse models have revealed its significant roles in diseases like AKI, DKD, and in regulating systemic insulin sensitivity. These findings provide potential therapeutic targets for these disease areas.
References:
1. Xiao, Ling, De Jesus, Dario F, Ju, Cheng-Wei, He, Chuan, Kulkarni, Rohit N. 2024. m6A mRNA methylation in brown fat regulates systemic insulin sensitivity via an inter-organ prostaglandin signaling axis independent of UCP1. In Cell metabolism, 36, 2207-2227.e9. doi:10.1016/j.cmet.2024.08.006. https://pubmed.ncbi.nlm.nih.gov/39255799/
2. Zhong, Dandan, Quan, Lingling, Hao, Chang, Jia, Zhanjun, Sun, Ying. 2023. Targeting mPGES-2 to protect against acute kidney injury via inhibition of ferroptosis dependent on p53. In Cell death & disease, 14, 710. doi:10.1038/s41419-023-06236-7. https://pubmed.ncbi.nlm.nih.gov/37907523/
3. Zhong, Dandan, Chen, Jingshuo, Qiao, Ranran, Jia, Zhanjun, Sun, Ying. 2024. Genetic or pharmacologic blockade of mPGES-2 attenuates renal lipotoxicity and diabetic kidney disease by targeting Rev-Erbα/FABP5 signaling. In Cell reports, 43, 114075. doi:10.1016/j.celrep.2024.114075. https://pubmed.ncbi.nlm.nih.gov/38583151/
4. Nakatani, Yoshihito, Kudo, Ichiro. . [Prostaglandin E2 synthases]. In Nihon yakurigaku zasshi. Folia pharmacologica Japonica, 120, 373-8. doi:. https://pubmed.ncbi.nlm.nih.gov/12528468/
5. Zhao, Zhibo, Liu, Xinyi, Xiang, Yue, Gong, Jianping, Zhao, Lei. 2023. Inhibiting cholesterol de novo synthesis promotes hepatocellular carcinoma progression by upregulating prostaglandin E synthase 2-mediated arachidonic acid metabolism under high fatty acid conditions. In Cancer science, 115, 477-489. doi:10.1111/cas.16035. https://pubmed.ncbi.nlm.nih.gov/38081591/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen