C57BL/6JCya-Tgfbrap1em1/Cya
Common Name:
Tgfbrap1-KO
Product ID:
S-KO-18727
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tgfbrap1-KO
Strain ID
KOCMP-73122-Tgfbrap1-B6J-VB
Gene Name
Product ID
S-KO-18727
Gene Alias
3110018K12Rik; Trap1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tgfbrap1em1/Cya mice (Catalog S-KO-18727) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000095014
NCBI RefSeq
NM_001013025
Target Region
Exon 5
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Tgfbrap1, also known as TGF-β receptor-associated binding protein 1, plays important roles in multiple biological processes. It is involved in the TGF-β signaling pathway, which is crucial for cell growth, differentiation, and tissue homeostasis [1,2,3,4,6,7,8]. Genetic studies on Tgfbrap1 can provide insights into its function and its implications in various diseases.
In pulmonary fibrosis, Chitinase 1 (CHIT1) regulates the TGF-β/SMAD7 pathway via interaction with Tgfbrap1 and FOXO3, highlighting its role in the pathogenesis of the disease [1,5,8]. In goose follicular granulosa cells, knockdown of Tgfbrap1 reduces apoptosis and enhances the secretion of E2 and P4, suggesting its role in regulating follicular development [2]. A variant at Tgfbrap1 is significantly associated with type 2 diabetes mellitus and affects diabetes-related miRNA expression, indicating its contribution to the genetic susceptibility of T2DM [3]. Also, the A variant of rs17687727 in Tgfbrap1 influences the susceptibility to antituberculosis drug-induced liver injury in the Han Chinese population [4]. In liver cancer, Tgfbrap1 stabilizes TGF-β receptor type 1, dictating feedback activation of the TGF-β signaling pathway to maintain liver cancer stemness and drug resistance [6]. Piperine, in protecting against acetaminophen-induced hepatotoxicity, may act through regulation of Tgfbrap1 [7]. Overexpression of Tgfbrap1, a CORVET-specific subunit, decreases the amount of assembled HOPS complex, affecting autophagosome-lysosome fusion and lysosomal hydrolase delivery [9].
In conclusion, Tgfbrap1 is a key player in the TGF-β signaling pathway and is involved in multiple biological processes such as tissue fibrosis, follicular development, and disease-related miRNA regulation. Its variants are associated with type 2 diabetes and antituberculosis drug-induced liver injury. The study of Tgfbrap1 in various in vivo models has provided valuable insights into its functions in these disease areas, contributing to our understanding of disease mechanisms and potential therapeutic targets.
References：
1. Lee, Chang-Min, He, Chuan-Hua, Park, Jin Wook, Elias, Jack A, Lee, Chun Geun. 2024. Retraction: Chitinase 1 regulates pulmonary fibrosis by modulating TGF-β/SMAD7 pathway via TGFBRAP1 and FOXO3. In Life science alliance, 7, . doi:10.26508/lsa.202402987. https://pubmed.ncbi.nlm.nih.gov/39209538/
2. Wang, Zhixiu, Lu, Lu, Gu, Tiantian, Xu, Qi, Chen, Guohong. 2020. The effects of FAR1 and TGFBRAP1 on the proliferation and apoptosis of follicular granulosa cells in goose (Anser cygnoides). In Gene, 769, 145194. doi:10.1016/j.gene.2020.145194. https://pubmed.ncbi.nlm.nih.gov/33007376/
3. Yang, Song, Chen, Xiaotian, Yang, Mengyao, Liu, Chunlan, Shen, Chong. 2018. The variant at TGFBRAP1 is significantly associated with type 2 diabetes mellitus and affects diabetes-related miRNA expression. In Journal of cellular and molecular medicine, 23, 83-92. doi:10.1111/jcmm.13885. https://pubmed.ncbi.nlm.nih.gov/30461200/
4. Zhang, Jingwei, Zhao, Zhenzhen, Bai, Hao, Lyv, Mengyuan, Ying, Binwu. 2019. The Variant at TGFBRAP1 but Not TGFBR2 Is Associated with Antituberculosis Drug-Induced Liver Injury. In Evidence-based complementary and alternative medicine : eCAM, 2019, 1685128. doi:10.1155/2019/1685128. https://pubmed.ncbi.nlm.nih.gov/31534460/
5. Lee, Chang-Min, He, Chuan-Hua, Park, Jin Wook, Elias, Jack A, Lee, Chun Geun. 2023. Correction: Chitinase 1 regulates pulmonary fibrosis by modulating TGF-β/SMAD7 pathway via TGFBRAP1 and FOXO3. In Life science alliance, 6, . doi:10.26508/lsa.202302065. https://pubmed.ncbi.nlm.nih.gov/37037591/
6. Liu, Kewei, Tian, Fanxuan, Chen, Xu, Wang, Tao, Wang, Bin. 2024. Stabilization of TGF-β Receptor 1 by a Receptor-Associated Adaptor Dictates Feedback Activation of the TGF-β Signaling Pathway to Maintain Liver Cancer Stemness and Drug Resistance. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2402327. doi:10.1002/advs.202402327. https://pubmed.ncbi.nlm.nih.gov/38981014/
7. Morsy, M A, Younis, N S, El-Sheikh, A A K, El-Daly, M, Mohafez, O M. . Protective mechanisms of piperine against acetaminophen-induced hepatotoxicity may be mediated through TGFBRAP1. In European review for medical and pharmacological sciences, 24, 10169-10180. doi:10.26355/eurrev_202010_23237. https://pubmed.ncbi.nlm.nih.gov/33090425/
8. Lee, Chang-Min, He, Chuan-Hua, Park, Jin Wook, Elias, Jack A, Lee, Chun Geun. 2019. Chitinase 1 regulates pulmonary fibrosis by modulating TGF-β/SMAD7 pathway via TGFBRAP1 and FOXO3. In Life science alliance, 2, . doi:10.26508/lsa.201900350. https://pubmed.ncbi.nlm.nih.gov/31085559/
9. Sőth, Ármin, Molnár, Márton, Lőrincz, Péter, Simon-Vecsei, Zsófia, Juhász, Gábor. 2024. CORVET-specific subunit levels determine the balance between HOPS/CORVET endosomal tethering complexes. In Scientific reports, 14, 10146. doi:10.1038/s41598-024-59775-0. https://pubmed.ncbi.nlm.nih.gov/38698024/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen