C57BL/6JCya-Mtfr1em1/Cya
Common Name
Mtfr1-KO
Product ID
S-KO-18736
Backgroud
C57BL/6JCya
Strain ID
KOCMP-67472-Mtfr1-B6J-VB
When using this mouse strain in a publication, please cite “Mtfr1-KO Mouse (Catalog S-KO-18736) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Mtfr1-KO
Strain ID
KOCMP-67472-Mtfr1-B6J-VB
Gene Name
Product ID
S-KO-18736
Gene Alias
1300002C08Rik, 1700080D04Rik, 2810026O10Rik, 4930579E05Rik, Chppr, Fam54a2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000130645
NCBI RefSeq
NM_026182
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Overview of Gene Research
MTFR1, or mitochondrial fission regulator 1, is a nuclear gene encoding a mitochondrial protein crucial for regulating mitochondrial dynamics [6,7]. It is involved in processes like mitochondrial fission, fusion, and respiration, and plays a role in maintaining mitochondrial function and cellular antioxidant activity [6,7]. It may also participate in various signaling pathways related to cell survival, metabolism, and proliferation [1,2,3,4].
MTFR1 has been shown to be highly expressed in multiple cancers. In tongue squamous cell carcinoma, its knockdown inhibits TGFβ1-induced epithelial-mesenchymal transition (EMT), migration, and invasion [1]. In lung adenocarcinoma, overexpression promotes cancer progression, drug-resistance to cisplatin, and is related to the immune microenvironment; interfering with its expression inhibits cell proliferation, migration, invasion, and promotes cisplatin sensitivity [2]. In colon cancer, MTFR1 phosphorylation during glucose deprivation promotes mitochondrial fusion and cell survival [3]. In addition, in cardiomyocytes, knocking down Mtfr1 suppresses mitochondrial fission, apoptosis, and myocardial infarction [5].
In conclusion, MTFR1 is essential for regulating mitochondrial dynamics and function, and its dysregulation is associated with various diseases, especially cancers. Studies using gene-knockdown or knockout models in different cell types and animal models have revealed its role in promoting cancer progression, drug-resistance, and in cardiac diseases, highlighting its potential as a therapeutic target.
References：
1. Jia, Yonglu, Chen, Xiaojuan, Zhao, Dayong, Ma, Sancheng. 2022. SNHG1/miR-194-5p/MTFR1 Axis Promotes TGFβ1-Induced EMT, Migration and Invasion of Tongue Squamous Cell Carcinoma Cells. In Molecular biotechnology, 64, 780-790. doi:10.1007/s12033-021-00445-1. https://pubmed.ncbi.nlm.nih.gov/35107755/
2. Li, Qian-Yun, Guo, Qiang, Luo, Wei-Min, Zhang, Jun, Ke, Di. 2024. Overexpression of MTFR1 promotes cancer progression and drug-resistance on cisplatin and is related to the immune microenvironment in lung adenocarcinoma. In Aging, 16, 66-88. doi:10.18632/aging.205338. https://pubmed.ncbi.nlm.nih.gov/38170222/
3. Zhang, Nan, Dong, Lu, Liu, Sifan, Ning, Tingting, Zhu, Shengtao. 2025. MTFR1 phosphorylation-activated adaptive mitochondrial fusion is essential for colon cancer cell survival during glucose deprivation. In Neoplasia (New York, N.Y.), 63, 101159. doi:10.1016/j.neo.2025.101159. https://pubmed.ncbi.nlm.nih.gov/40121946/
4. Li, Yongmeng, Liu, Yanfei, Jin, Kai, Zhang, Huiying, Tian, Hui. 2021. Negatively Regulated by miR-29c-3p, MTFR1 Promotes the Progression and Glycolysis in Lung Adenocarcinoma via the AMPK/mTOR Signalling Pathway. In Frontiers in cell and developmental biology, 9, 771824. doi:10.3389/fcell.2021.771824. https://pubmed.ncbi.nlm.nih.gov/34926459/
5. Wang, K, Zhang, D-L, Long, B, Liu, C-Y, Li, P-F. 2015. NFAT4-dependent miR-324-5p regulates mitochondrial morphology and cardiomyocyte cell death by targeting Mtfr1. In Cell death & disease, 6, e2007. doi:10.1038/cddis.2015.348. https://pubmed.ncbi.nlm.nih.gov/26633713/
6. Monticone, Massimiliano, Panfoli, Isabella, Ravera, Silvia, Boitani, Carla, Castagnola, Patrizio. . The nuclear genes Mtfr1 and Dufd1 regulate mitochondrial dynamic and cellular respiration. In Journal of cellular physiology, 225, 767-76. doi:10.1002/jcp.22279. https://pubmed.ncbi.nlm.nih.gov/20568109/
7. Monticone, Massimiliano, Tonachini, Laura, Tavella, Sara, Cancedda, Ranieri, Castagnola, Patrizio. . Impaired expression of genes coding for reactive oxygen species scavenging enzymes in testes of Mtfr1/Chppr-deficient mice. In Reproduction (Cambridge, England), 134, 483-92. doi:. https://pubmed.ncbi.nlm.nih.gov/17709566/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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