C57BL/6JCya-Myl3em1/Cya
Common Name:
Myl3-KO
Product ID:
S-KO-18738
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Myl3-KO
Strain ID
KOCMP-17897-Myl3-B6J-VB
Gene Name
Product ID
S-KO-18738
Gene Alias
MLC1SB; MLC1s; MLC1v; Mylc; VLC1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Myl3em1/Cya mice (Catalog S-KO-18738) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000079784
NCBI RefSeq
NM_010859
Target Region
Exon 2~4
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
MYL3, also known as myosin light chain 3, is a gene encoding a sarcomeric protein. Sarcomeric proteins are essential for muscle contraction, and thus, MYL3 is crucial for normal muscle function. Mutations in this gene have been associated with hypertrophic cardiomyopathy and other related cardiac diseases, highlighting its significance in cardiac health [2,3,4,5,6,7,8,9].
In chondrocytes, MYL3 has been shown to play a protective role against senescence. Conditional deletion of Myl3 in chondrocytes of male mice significantly promoted osteoarthritis (OA) progression, while intra-articular injection of adeno-associated virus overexpressing MYL3 delayed OA progression. MYL3 deficiency enhanced clathrin-mediated endocytosis by promoting the interaction between myosin VI and clathrin, leading to the internalization of Notch and activation of Notch signaling in chondrocytes. Blocking this signaling pathway prevented MYL3 loss-induced chondrocyte senescence and alleviated OA progression [1].
In conclusion, MYL3 is essential for maintaining normal muscle function, especially in the context of cardiac health and chondrocyte senescence. Studies using gene knockout and overexpression models in mice have revealed its role in inhibiting chondrocyte senescence and thus potentially preventing OA development. In the context of cardiomyopathies, understanding MYL3 mutations can contribute to better diagnosis and management of hypertrophic cardiomyopathy.
References:
1. Cao, He, Yang, Panpan, Liu, Jia, Bai, Xiaochun, Li, Kai. 2023. MYL3 protects chondrocytes from senescence by inhibiting clathrin-mediated endocytosis and activating of Notch signaling. In Nature communications, 14, 6190. doi:10.1038/s41467-023-41858-7. https://pubmed.ncbi.nlm.nih.gov/37794006/
2. Berge, K E, Leren, T P. 2013. Genetics of hypertrophic cardiomyopathy in Norway. In Clinical genetics, 86, 355-60. doi:10.1111/cge.12286. https://pubmed.ncbi.nlm.nih.gov/24111713/
3. Ingles, Jodie, Goldstein, Jennifer, Thaxton, Courtney, Hershberger, Ray E, Funke, Birgit. . Evaluating the Clinical Validity of Hypertrophic Cardiomyopathy Genes. In Circulation. Genomic and precision medicine, 12, e002460. doi:10.1161/CIRCGEN.119.002460. https://pubmed.ncbi.nlm.nih.gov/30681346/
4. Akinrinade, Oyediran, Lesurf, Robert, Lougheed, Jane, Oechslin, Erwin, Mital, Seema. 2023. Age and Sex Differences in the Genetics of Cardiomyopathy. In Journal of cardiovascular translational research, 16, 1287-1302. doi:10.1007/s12265-023-10411-8. https://pubmed.ncbi.nlm.nih.gov/37477868/
5. Mavilakandy, Akash, Ahamed, Hisham. 2022. Mutation of the MYL3 gene in a patient with mid-ventricular obstructive hypertrophic cardiomyopathy. In BMJ case reports, 15, . doi:10.1136/bcr-2021-244573. https://pubmed.ncbi.nlm.nih.gov/35288424/
6. Abbas, Mohammed Tiseer, Baba Ali, Nima, Farina, Juan M, Ayoub, Chadi, Arsanjani, Reza. 2024. Role of Genetics in Diagnosis and Management of Hypertrophic Cardiomyopathy: A Glimpse into the Future. In Biomedicines, 12, . doi:10.3390/biomedicines12030682. https://pubmed.ncbi.nlm.nih.gov/38540296/
7. Topriceanu, Constantin-Cristian, Pereira, Alexandre C, Moon, James C, Captur, Gabriella, Ho, Carolyn Y. 2023. Meta-Analysis of Penetrance and Systematic Review on Transition to Disease in Genetic Hypertrophic Cardiomyopathy. In Circulation, 149, 107-123. doi:10.1161/CIRCULATIONAHA.123.065987. https://pubmed.ncbi.nlm.nih.gov/37929589/
8. Gómez, Juan, Lorca, Rebeca, Reguero, Julian R, Avanzas, Pablo, Coto, Eliecer. . Screening of the Filamin C Gene in a Large Cohort of Hypertrophic Cardiomyopathy Patients. In Circulation. Cardiovascular genetics, 10, . doi:10.1161/CIRCGENETICS.116.001584. https://pubmed.ncbi.nlm.nih.gov/28356264/
9. Osborn, Daniel Peter Sayer, Emrahi, Leila, Clayton, Joshua, Jamshidi, Yalda, Tajsharghi, Homa. 2020. Autosomal recessive cardiomyopathy and sudden cardiac death associated with variants in MYL3. In Genetics in medicine : official journal of the American College of Medical Genetics, 23, 787-792. doi:10.1038/s41436-020-01028-2. https://pubmed.ncbi.nlm.nih.gov/33288880/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen