C57BL/6NCya-Ildr2em1/Cya
Common Name:
Ildr2-KO
Product ID:
S-KO-18744
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ildr2-KO
Strain ID
KOCMP-100039795-Ildr2-B6N-VA
Gene Name
Product ID
S-KO-18744
Gene Alias
2810478N18Rik; 3110063L10Rik; D1Ertd471e; Dbsm1; Ll
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ildr2em1/Cya mice (Catalog S-KO-18744) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000111416
NCBI RefSeq
NM_001164528
Target Region
Exon 7~8
Size of Effective Region
~5.9 kb
Detailed Document
Overview of Gene Research
Ildr2, also known as angulin-3, is a member of the Ig superfamily and is implicated in tricellular tight junctions. It has a role in pancreatic islet health, immune regulation, and hepatic lipid homeostasis. In pancreatic β -cells, it may be involved in a new pathway related to hypoinsulinemic hyperglycemia [1]. In the immune system, it can deliver inhibitory signals to T cells, and ILDR2-Fc shows potential in treating autoimmune diseases by regulating immune homeostasis and inducing antigen-specific immune tolerance [3,4].
In gene-knockout studies, liver-specific Ildr2 knockout mice did not develop hepatic steatosis, indicating that previous observations of hepatic steatosis in Ildr2 knockdown mice were likely due to "off-target" effects of shRNA [2]. In Ildr2-knockout mice related to glomerulopathies, there was glomerular hypertrophy and decreased podocyte density, suggesting a protective role of ILDR2 in glomerulopathies [5].
In conclusion, Ildr2 is crucial in multiple biological processes such as pancreatic function, immune regulation, and lipid and glomerular homeostasis. Gene-knockout mouse models have been instrumental in clarifying its role in hepatic steatosis and glomerulopathies, providing insights into potential therapeutic targets for related diseases.
References：
1. Watanabe, Kazuhisa, Nakayama, Kazuhiro, Ohta, Satoshi, Tsuda, Hidetoshi, Iwamoto, Sadahiko. 2021. ILDR2 stabilization is regulated by its interaction with GRP78. In Scientific reports, 11, 8414. doi:10.1038/s41598-021-87884-7. https://pubmed.ncbi.nlm.nih.gov/33863978/
2. Millings, Elizabeth J, De Rosa, Maria Caterina, Fleet, Sarah, Fischer, Stuart G, Leibel, Rudolph L. 2018. ILDR2 has a negligible role in hepatic steatosis. In PloS one, 13, e0197548. doi:10.1371/journal.pone.0197548. https://pubmed.ncbi.nlm.nih.gov/29847571/
3. Podojil, Joseph R, Hecht, Iris, Chiang, Ming-Yi, Rotman, Galit, Miller, Stephen D. 2018. ILDR2-Fc Is a Novel Regulator of Immune Homeostasis and Inducer of Antigen-Specific Immune Tolerance. In Journal of immunology (Baltimore, Md. : 1950), 200, 2013-2024. doi:10.4049/jimmunol.1700326. https://pubmed.ncbi.nlm.nih.gov/29431690/
4. Hecht, Iris, Toporik, Amir, Podojil, Joseph R, Neria, Eyal, Rotman, Galit. 2018. ILDR2 Is a Novel B7-like Protein That Negatively Regulates T Cell Responses. In Journal of immunology (Baltimore, Md. : 1950), 200, 2025-2037. doi:10.4049/jimmunol.1700325. https://pubmed.ncbi.nlm.nih.gov/29431694/
5. Siegerist, Florian, Kliewe, Felix, Hammer, Elke, Völker, Uwe, Endlich, Nicole. 2024. The role of the tricellular junction protein ILDR2 in glomerulopathies: Expression patterns and functional insights. In iScience, 27, 111329. doi:10.1016/j.isci.2024.111329. https://pubmed.ncbi.nlm.nih.gov/39640577/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen