C57BL/6JCya-Golga3em1/Cya
Common Name:
Golga3-KO
Product ID:
S-KO-18745
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Golga3-KO
Strain ID
KOCMP-269682-Golga3-B6J-VA
Gene Name
Product ID
S-KO-18745
Gene Alias
5330413L04; 5430416E01Rik; G1-499-14; Mea-2; Mea2; Mea2/Golga3; repro27
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Golga3em1/Cya mice (Catalog S-KO-18745) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031477
NCBI RefSeq
NM_001347389
Target Region
Exon 6~9
Size of Effective Region
~5.9 kb
Detailed Document
Overview of Gene Research
Golga3, also known as Mea2, is a member of the golgin subfamily A. It is a Golgi complex-associated protein involved in protein transport, cell apoptosis, Golgi localization, and spermatogenesis [1,2,3]. It has eight phosphorylation sites, and its function may also be related to pathways like ubiquitin-dependent proteasomal degradation as it interacts with serine racemase [4]. Genetic mouse models are valuable for studying its functions.
In mouse models, Golga3 knockout (KO) male mice show infertility [2]. In Golga3(repro27) mutant mice with a nonsense mutation in Golga3, spermatogenesis initiates normally but is disrupted in late meiosis, leading to germ cell loss, abnormal spermiogenesis, and low sperm concentration and motility [2]. However, mutation of the S461 phosphorylation site in Golga3 does not affect mouse spermatogenesis [1]. Also, in a transgenic mouse where Golga3/Mea2 was disrupted, a truncated form (DeltaMea2) restored spermatogenesis in a dose-dependent manner, suggesting the importance of Golga3 for pachytene spermatocyte survival [3].
In conclusion, Golga3 is crucial for spermatogenesis as demonstrated by KO mouse models. Defects in Golga3 lead to male infertility due to disrupted spermatogenesis. These models have enhanced our understanding of the role of Golga3 in male germ cell development and may have implications for understanding human male infertility [1,2,3].
References:
1. Xu, Changtong, Zhu, Mingcong, Zhao, Shuqin, Wang, Ying, Liu, Mingxi. 2023. Mutation of S461, in the GOLGA3 phosphorylation site, does not affect mouse spermatogenesis. In PeerJ, 11, e15133. doi:10.7717/peerj.15133. https://pubmed.ncbi.nlm.nih.gov/37090114/
2. Bentson, L F, Agbor, V A, Agbor, L N, Handel, M A, Linder, C C. 2013. New point mutation in Golga3 causes multiple defects in spermatogenesis. In Andrology, 1, 440-50. doi:10.1111/j.2047-2927.2013.00070.x. https://pubmed.ncbi.nlm.nih.gov/23495255/
3. Banu, Yasmin, Matsuda, Miyuki, Yoshihara, Mitsuyo, Sutou, Sizuyo, Matsukuma, Shoichi. . Golgi matrix protein gene, Golga3/Mea2, rearranged and re-expressed in pachytene spermatocytes restores spermatogenesis in the mouse. In Molecular reproduction and development, 61, 288-301. doi:. https://pubmed.ncbi.nlm.nih.gov/11835574/
4. Dumin, Elena, Bendikov, Inna, Foltyn, Veronika N, Kartvelishvily, Elena, Wolosker, Herman. 2006. Modulation of D-serine levels via ubiquitin-dependent proteasomal degradation of serine racemase. In The Journal of biological chemistry, 281, 20291-302. doi:. https://pubmed.ncbi.nlm.nih.gov/16714286/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen