2700049A03Rik, also known as Talpid3 in mouse (KIAA0856 in human), is a gene encoding a centrosomal protein essential for the assembly of primary cilia [2]. It is associated with pathways related to cilia-mediated functions, and its study through genetic models is crucial for understanding related biological processes.

In a study on renal ischemia reperfusion injury, the promoter methylation of 2700049A03Rik was found to be altered following renal injury. Its promoter methylation was negatively correlated with its mRNA expression in renal tissues, indicating that promoter methylation may be an important regulatory mechanism for its expression [1].

In a conditional knockout (CKO) mouse model of Joubert syndrome, deletion of conserved exons 11-12 of Talpid3 (2700049A03Rik) in the central nervous system recapitulated the complete cerebellar phenotype of the disease. These mice showed key hallmarks of Joubert syndrome, including progressive ataxia, cerebellar hypoplasia, and abnormal Purkinje cell and granule cell development, suggesting a role for 2700049A03Rik in granule precursor cell migration in the cerebellum, potentially through both Hedgehog and Wnt signaling pathways [2].

In conclusion, 2700049A03Rik is essential for primary cilia assembly. Its role in renal ischemia reperfusion injury is related to promoter-methylation-mediated gene expression. In Joubert syndrome, CKO mouse models have revealed its role in cerebellar development, contributing to our understanding of the disease mechanism. Overall, these model-based studies enhance our knowledge of 2700049A03Rik's biological functions and its implications in disease.