C57BL/6JCya-Cldn5em1/Cya
Common Name:
Cldn5-KO
Product ID:
S-KO-18752
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cldn5-KO
Strain ID
KOCMP-12741-Cldn5-B6J-VA
Gene Name
Product ID
S-KO-18752
Gene Alias
MBEC1; Tmvcf
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cldn5em1/Cya mice (Catalog S-KO-18752) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000043577
NCBI RefSeq
NM_013805
Target Region
Exon 1
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Cldn5, also known as claudin-5, is an essential component of tight junctions. It is crucial for the integrity of the blood-brain barrier (BBB), ensuring the homeostasis and protection of the central nervous system (CNS) from damage. It limits the passive diffusions of ions and solutes in endothelial cells [1,2].
In podocyte-specific Cldn5 knockout mice, Cldn5 deletion exacerbates podocyte injury and proteinuria in a diabetic nephropathy mouse model. Mechanistically, it reduces ZO1 expression, induces nuclear translocation of ZONAB, down-regulates WNT inhibitory factor-1 (WIF1) expression, and activates the WNT signaling pathway [4]. In zebrafish models, mutations in Cldn5 analogous to those in patients result in a novel aberrant function, causing a neurodevelopmental disorder involving BBB disruption and impaired neuronal function [3].
In conclusion, Cldn5 is vital for maintaining the integrity of the BBB and has a significant impact on CNS-related diseases. The study of Cldn5 knockout models in mice and zebrafish has revealed its role in diseases such as neurodevelopmental disorders and kidney diseases, providing insights into the underlying mechanisms and potential therapeutic targets.
References：
1. Ling, Yao, Kang, Xinxin, Yi, Ying, Ma, Guanshen, Qu, Huinan. 2024. CLDN5: From structure and regulation to roles in tumors and other diseases beyond CNS disorders. In Pharmacological research, 200, 107075. doi:10.1016/j.phrs.2024.107075. https://pubmed.ncbi.nlm.nih.gov/38228255/
2. Hashimoto, Yosuke, Greene, Chris, Munnich, Arnold, Campbell, Matthew. 2023. The CLDN5 gene at the blood-brain barrier in health and disease. In Fluids and barriers of the CNS, 20, 22. doi:10.1186/s12987-023-00424-5. https://pubmed.ncbi.nlm.nih.gov/36978081/
3. Deshwar, Ashish R, Cytrynbaum, Cheryl, Murthy, Harsha, Dowling, James J, Weksberg, Rosanna. . Variants in CLDN5 cause a syndrome characterized by seizures, microcephaly and brain calcifications. In Brain : a journal of neurology, 146, 2285-2297. doi:10.1093/brain/awac461. https://pubmed.ncbi.nlm.nih.gov/36477332/
4. Sun, Hui, Li, Hui, Yan, Jie, Zhao, Shengtian, Gong, Yongfeng. 2022. Loss of CLDN5 in podocytes deregulates WIF1 to activate WNT signaling and contributes to kidney disease. In Nature communications, 13, 1600. doi:10.1038/s41467-022-29277-6. https://pubmed.ncbi.nlm.nih.gov/35332151/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen