C57BL/6JCya-Gpat4em1/Cya
Common Name:
Gpat4-KO
Product ID:
S-KO-18766
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gpat4-KO
Strain ID
KOCMP-102247-Gpat4-B6J-VA
Gene Name
Product ID
S-KO-18766
Gene Alias
Agpat6; Tsarg7
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpat4em1/Cya mice (Catalog S-KO-18766) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000167004
NCBI RefSeq
NM_018743
Target Region
Exon 4
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Gpat4, glycerol-3-phosphate acyltransferase 4, is an endoplasmic reticulum membrane-anchored protein. It is crucial in glycerophospholipid biosynthesis and plays a part in lipid-related metabolic pathways. In plants, land-plant-specific GPATs, including those in the GPAT4/6/8 clade, are central to cutin and suberin monomer biosynthesis [1,3]. In mammals, it is involved in processes like adipogenesis and regulation of fatty acid metabolism [4,5]. Genetic models, such as knockout mice, have been valuable in studying its functions.
In Arabidopsis, the GPAT4/6/8 clade, along with the GPAT5/7 clade, is required for root suberization. GPAT4 has an additive effect with GPAT6 in root suberin biosynthesis and a redundant role with GPAT8 in wax production [1,3]. In mice, Gpat4 global and tissue-specific knockout models have shown that its deficiency in endocardial cells provokes ER stress response, enhances ER-mit communications, and triggers the cGAS-STING pathway, affecting heart development [2]. Gpat4-null pups had growth issues during suckling, and adult mice were protected from high-fat-diet-induced obesity. In brown adipocytes, Gpat4 limits oxidation of exogenous fatty acids [4]. Also, in 3T3-L1 adipocytes, shRNA-mediated knockdown of GPAT4 did not significantly decrease GPAT activity or inhibit lipid accumulation during adipocyte differentiation as much as GPAT3 knockdown, suggesting distinct roles for GPAT4 in adipogenesis [5]. In a high-fat-diet mouse model, hippocampal GPAT4 expression was up-regulated, and it may participate in diet-induced depression through the AMPK/CREB/BDNF pathway [6].
In conclusion, Gpat4 is essential for lipid-related biological processes in both plants and animals. In animals, studies using knockout mouse models have revealed its significance in heart development, obesity-related metabolism, adipogenesis, and the development of obesity-induced depression. These findings contribute to understanding the underlying mechanisms of related diseases and may provide potential targets for intervention.
References：
1. Gully, Kay, Berhin, Alice, De Bellis, Damien, Feussner, Ivo, Nawrath, Christiane. 2024. The GPAT4/6/8 clade functions in Arabidopsis root suberization nonredundantly with the GPAT5/7 clade required for suberin lamellae. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2314570121. doi:10.1073/pnas.2314570121. https://pubmed.ncbi.nlm.nih.gov/38739804/
2. Zhao, Tianyang, Jin, Kuipei, Wang, Xiaodong, Yang, Hongyuan, Yang, Zhongzhou. 2025. GPAT4 sustains endoplasmic reticulum homeostasis in endocardial cells and safeguards heart development. In Nature communications, 16, 3345. doi:10.1038/s41467-025-58722-5. https://pubmed.ncbi.nlm.nih.gov/40199910/
3. Zhang, Xuanhao, Gao, Huani, Liu, Yi, Zhao, Huayan, Lü, Shiyou. 2023. Function identification of Arabidopsis GPAT4 and GPAT8 in the biosynthesis of suberin and cuticular wax. In Plant science : an international journal of experimental plant biology, 339, 111933. doi:10.1016/j.plantsci.2023.111933. https://pubmed.ncbi.nlm.nih.gov/38036221/
4. Cooper, Daniel E, Grevengoed, Trisha J, Klett, Eric L, Coleman, Rosalind A. 2015. Glycerol-3-phosphate Acyltransferase Isoform-4 (GPAT4) Limits Oxidation of Exogenous Fatty Acids in Brown Adipocytes. In The Journal of biological chemistry, 290, 15112-20. doi:10.1074/jbc.M115.649970. https://pubmed.ncbi.nlm.nih.gov/25918168/
5. Shan, Dandan, Li, Jian-liang, Wu, Leeying, Gimeno, Ruth E, Cao, Jingsong. 2010. GPAT3 and GPAT4 are regulated by insulin-stimulated phosphorylation and play distinct roles in adipogenesis. In Journal of lipid research, 51, 1971-81. doi:10.1194/jlr.M006304. https://pubmed.ncbi.nlm.nih.gov/20181984/
6. Huang, Yin-Qiong, Wang, Yaofeng, Hu, Keyue, Lin, Shu, Lin, Xia-Hong. 2021. Hippocampal Glycerol-3-Phosphate Acyltransferases 4 and BDNF in the Progress of Obesity-Induced Depression. In Frontiers in endocrinology, 12, 667773. doi:10.3389/fendo.2021.667773. https://pubmed.ncbi.nlm.nih.gov/34054732/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen