C57BL/6JCya-Satb2em1/Cya
Common Name:
Satb2-KO
Product ID:
S-KO-18767
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Satb2-KO
Strain ID
KOCMP-212712-Satb2-B6J-VA
Gene Name
Product ID
S-KO-18767
Gene Alias
BAP002; mKIAA1034
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Satb2em1/Cya mice (Catalog S-KO-18767) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000114415
NCBI RefSeq
NM_139146
Target Region
Exon 7
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
SATB2, short for special AT-rich binding protein-2, is a member of the matrix attachment region-binding transcription factor family. It plays crucial roles in multiple biological processes, including craniofacial, neural, and osteoblastic differentiation. SATB2 modulates chromatin architecture to regulate gene expression and is involved in pathways related to cell pluripotency, self-renewal, and epithelial-mesenchymal transition (EMT) [2,3,4].
In mouse models, Satb2 loss in adult mice leads to the stable conversion of colonic stem cells into small intestine ileal-like stem cells, replacing the colonic mucosa with an ileum-like one. Conversely, SATB2 can confer colonic properties on the mouse ileum. This shows that SATB2 singularly preserves LGR5+ adult colonic stem cell and epithelial identity in mice and humans, mediating cross-tissue plasticity in mature intestines [1].
In conclusion, SATB2 is a versatile transcriptional regulator. Its functions span across development, from craniofacial and skeleton formation to neural development. In disease, it has implications in cancer, especially colorectal cancer where it may serve as a biomarker and potential therapeutic target. Mouse models, particularly those with Satb2 knockout or conditional knockout, have been instrumental in uncovering its role in maintaining colonic stem cell identity and understanding its implications in intestinal tissue plasticity and cancer-related processes [1,2].
References:
1. Gu, Wei, Wang, Hua, Huang, Xiaofeng, Shivdasani, Ramesh A, Zhou, Qiao. 2021. SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling. In Cell stem cell, 29, 101-115.e10. doi:10.1016/j.stem.2021.09.004. https://pubmed.ncbi.nlm.nih.gov/34582804/
2. Roy, Sanjit K, Shrivastava, Anju, Srivastav, Sudesh, Shankar, Sharmila, Srivastava, Rakesh K. 2020. SATB2 is a novel biomarker and therapeutic target for cancer. In Journal of cellular and molecular medicine, 24, 11064-11069. doi:10.1111/jcmm.15755. https://pubmed.ncbi.nlm.nih.gov/32885593/
3. Chen, Qiao Yi, Des Marais, Thomas, Costa, Max. . Deregulation of SATB2 in carcinogenesis with emphasis on miRNA-mediated control. In Carcinogenesis, 40, 393-402. doi:10.1093/carcin/bgz020. https://pubmed.ncbi.nlm.nih.gov/30916759/
4. Huang, Xia, Chen, Qiuman, Luo, Wenping, El Dafrawy, Mostafa, Zhang, Hongmei. 2020. SATB2: A versatile transcriptional regulator of craniofacial and skeleton development, neurogenesis and tumorigenesis, and its applications in regenerative medicine. In Genes & diseases, 9, 95-107. doi:10.1016/j.gendis.2020.10.003. https://pubmed.ncbi.nlm.nih.gov/35005110/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen