C57BL/6JCya-Clpbem1/Cya
Common Name:
Clpb-KO
Product ID:
S-KO-18772
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Clpb-KO
Strain ID
KOCMP-20480-Clpb-B6J-VA
Gene Name
Product ID
S-KO-18772
Gene Alias
Skd3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Clpbem1/Cya mice (Catalog S-KO-18772) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001884
NCBI RefSeq
NM_009191
Target Region
Exon 4
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
ClpB, a member of class 1 AAA+ proteins, is a key chaperone with disaggregase activity. It plays a crucial role in bacterial survival under various stress conditions, such as heat shock, and is also involved in regulating the secretion of bacterial effector molecules related to type VI secretion systems [1]. In plants, it is essential for heat stress tolerance, and in bacteria, it cooperates with the DnaK chaperone system to reactivate aggregated proteins and promote survival under adverse conditions [3,4,5]. Since ClpB is not present in human cells, it is an attractive target for antimicrobial therapies [3].
CLPB mutations are associated with 3-methylglutaconic aciduria and neutropenia. In a CLPB-null environment, there is a stress-specific aggregation phenotype, and CLPB deficiency impedes the function of the proteolytic SPY complex due to protein aggregation in the mitochondrial intermembrane space, indicating an interdependency of mitochondrial quality control components at the intermembrane space/inner membrane interface [2].
In conclusion, ClpB is vital for stress responses in bacteria and plants, with its disaggregase activity being key to protein reactivation and survival under stress. In humans, mutations in CLPB are associated with neutropenia and mitochondrial-related diseases. Studies on ClpB provide insights into bacterial virulence, plant stress tolerance, and human mitochondrial chaperonopathies, potentially guiding the development of novel antimicrobial therapies and understanding of related disease mechanisms.
References:
1. Alam, Athar, Bröms, Jeanette E, Kumar, Rajender, Sjöstedt, Anders. 2021. The Role of ClpB in Bacterial Stress Responses and Virulence. In Frontiers in molecular biosciences, 8, 668910. doi:10.3389/fmolb.2021.668910. https://pubmed.ncbi.nlm.nih.gov/33968993/
2. Baker, Megan J, Blau, Kai Uwe, Anderson, Alexander J, Langer, Thomas, Stojanovski, Diana. 2024. CLPB disaggregase dysfunction impacts the functional integrity of the proteolytic SPY complex. In The Journal of cell biology, 223, . doi:10.1083/jcb.202305087. https://pubmed.ncbi.nlm.nih.gov/38270563/
3. Kędzierska-Mieszkowska, Sabina, Zolkiewski, Michal. 2021. Hsp100 Molecular Chaperone ClpB and Its Role in Virulence of Bacterial Pathogens. In International journal of molecular sciences, 22, . doi:10.3390/ijms22105319. https://pubmed.ncbi.nlm.nih.gov/34070174/
4. Mishra, Ratnesh Chandra, Grover, Anil. 2015. ClpB/Hsp100 proteins and heat stress tolerance in plants. In Critical reviews in biotechnology, 36, 862-74. doi:10.3109/07388551.2015.1051942. https://pubmed.ncbi.nlm.nih.gov/26121931/
5. Kędzierska-Mieszkowska, Sabina, Arent, Zbigniew. 2020. AAA+ Molecular Chaperone ClpB in Leptospira interrogans: Its Role and Significance in Leptospiral Virulence and Pathogenesis of Leptospirosis. In International journal of molecular sciences, 21, . doi:10.3390/ijms21186645. https://pubmed.ncbi.nlm.nih.gov/32932775/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen