C57BL/6JCya-Pdpk1em1/Cya
Common Name:
Pdpk1-KO
Product ID:
S-KO-18774
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Pdpk1-KO
Strain ID
KOCMP-18607-Pdpk1-B6J-VA
Gene Name
Product ID
S-KO-18774
Gene Alias
Pdk1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pdpk1em1/Cya mice (Catalog S-KO-18774) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102927
NCBI RefSeq
NM_011062
Target Region
Exon 3~4
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Pdpk1, also known as 3-phosphoinositide dependent protein kinase 1, is a phosphorylation-regulated kinase. It plays a central role in activating multiple signaling pathways, such as the AKT1-MTOR pathway, and is involved in various cellular processes like autophagy, cell proliferation, and metabolism [1,2,4,5].
In autophagy, Pdpk1 can act as a SUMOylation-regulated kinase to turn on macroautophagy. SUMOylated Pdpk1 regulates its phosphorylation and AKT1-MTOR pathway activation, while non-SUMOylated Pdpk1 tethers LC3 to the endoplasmic reticulum to initiate autophagy [1]. In hepatocellular carcinoma, AQP3 accumulation induced by FBXW5 knockdown leads to Pdpk1 degradation, inactivating the AKT-MTOR pathway and inducing autophagic death [2]. Also, Pdpk1 phosphorylation of SQSTM1 at T138 by coronavirus M protein promotes mitophagy over virophagy, and a Pdpk1-targeting peptide can inhibit RNA virus replication [3].
In conclusion, Pdpk1 is crucial in regulating autophagy-related processes and has implications in virus-host interactions and cancer-related autophagic cell death. Understanding Pdpk1's function through in vivo models like those in the studies provides insights into potential therapeutic targets for treating virus-induced diseases and certain cancers.
References:
1. Hu, Boli, Zhang, Yina, Deng, Tingjuan, Jin, Yulan, Zhou, Jiyong. 2020. PDPK1 regulates autophagosome biogenesis by binding to PIK3C3. In Autophagy, 17, 2166-2183. doi:10.1080/15548627.2020.1817279. https://pubmed.ncbi.nlm.nih.gov/32876514/
2. Liang, Yupei, Chen, Ping, Wang, Shiwen, Wei, Wenyi, Jia, Lijun. 2024. SCFFBXW5-mediated degradation of AQP3 suppresses autophagic cell death through the PDPK1-AKT-MTOR axis in hepatocellular carcinoma cells. In Autophagy, 20, 1984-1999. doi:10.1080/15548627.2024.2353497. https://pubmed.ncbi.nlm.nih.gov/38726865/
3. Li, Yahui, Li, Chunyan, Zhao, Chenchen, Zhou, Jiyong, Hu, Boli. 2024. Coronavirus M protein promotes mitophagy over virophagy by recruiting PDPK1 to phosphorylate SQSTM1 at T138. In Nature communications, 15, 8927. doi:10.1038/s41467-024-53100-z. https://pubmed.ncbi.nlm.nih.gov/39414765/
4. Sun, Fei, Wang, Fa-Xi, Zhu, He, Zhang, Shu, Wang, Cong-Yi. 2022. SUMOylation of PDPK1 Is required to maintain glycolysis-dependent CD4 T-cell homeostasis. In Cell death & disease, 13, 181. doi:10.1038/s41419-022-04622-1. https://pubmed.ncbi.nlm.nih.gov/35210408/
5. Liu, Shufeng, Chen, Xiaoguang, Zhang, Liqi, Lu, Bo. 2024. CPT1A mediates the succinylation of SP5 which activates transcription of PDPK1 to promote the viability and glycolysis of prostate cancer cells. In Cancer biology & therapy, 25, 2329372. doi:10.1080/15384047.2024.2329372. https://pubmed.ncbi.nlm.nih.gov/38494680/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen