C57BL/6JCya-Rragaem1/Cya
Common Name:
Rraga-KO
Product ID:
S-KO-18778
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Rraga-KO
Strain ID
KOCMP-68441-Rraga-B6J-VA
Gene Name
Product ID
S-KO-18778
Gene Alias
1300010C19Rik; FIP-1; RAGA
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rragaem1/Cya mice (Catalog S-KO-18778) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000091064
NCBI RefSeq
NM_178376
Target Region
Exon 1
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
RRAGA, encoding Ras-related GTP-binding protein A (RagA), is a key regulator in the mechanistic target of rapamycin complex 1 (mTORC1) pathway. RagA initially senses cellular amino acids (e.g., leucine) and controls mTORC1's translocation to the lysosomal membrane. This pathway is crucial for regulating cell growth and metabolism in response to environmental cues, including nutrients [1,2,4]. Genetic models, such as gene knockout, are valuable for studying its functions.
In a study generating a RRAGA knockout human iPSC line, it was aimed to discover the biological roles of RagA, potentially helping develop new therapeutics for depression [1]. In another functional study, mutations of RRAGA associated with autosomal dominant cataracts disrupted mTORC1 signaling in human lens epithelial cells, with effects like increased RRAGA relocation to lysosomes, up-regulated mTORC1 phosphorylation, down-regulated autophagy, and altered cell growth [3].
In conclusion, RRAGA plays an essential role in the mTORC1 pathway by sensing amino acids and regulating mTORC1 translocation. Studies using gene knockout models have provided insights into its role in diseases such as depression and autosomal dominant cataracts, highlighting its potential as a therapeutic target for these conditions.
References:
1. Sun, Yilu, Fu, Jian, Yang, Jiayin, Zhao, Jia, Rong, Jianhui. 2022. Generation of a RRAGA knockout human iPSC line GIBHi002-A-5 using Nuclease technology technology. In Stem cell research, 63, 102859. doi:10.1016/j.scr.2022.102859. https://pubmed.ncbi.nlm.nih.gov/35870248/
2. Valenstein, Max L, Lalgudi, Pranav V, Gu, Xin, Chivukula, Raghu R, Sabatini, David M. 2024. Rag-Ragulator is the central organizer of the physical architecture of the mTORC1 nutrient-sensing pathway. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2322755121. doi:10.1073/pnas.2322755121. https://pubmed.ncbi.nlm.nih.gov/39163330/
3. Chen, Jian-Huan, Huang, Chukai, Zhang, Bining, Pang, Chi-Pui, Zhang, Mingzhi. 2016. Mutations of RagA GTPase in mTORC1 Pathway Are Associated with Autosomal Dominant Cataracts. In PLoS genetics, 12, e1006090. doi:10.1371/journal.pgen.1006090. https://pubmed.ncbi.nlm.nih.gov/27294265/
4. Kim, Joungmok, Kim, Eunjung. 2016. Rag GTPase in amino acid signaling. In Amino acids, 48, 915-928. doi:10.1007/s00726-016-2171-x. https://pubmed.ncbi.nlm.nih.gov/26781224/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen