C57BL/6JCya-Cpne7em1/Cya
Common Name:
Cpne7-KO
Product ID:
S-KO-18792
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Contact for Pricing
Basic Information
Strain Name
Cpne7-KO
Strain ID
KOCMP-102278-Cpne7-B6J-VC
Gene Name
Product ID
S-KO-18792
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cpne7em1/Cya mice (Catalog S-KO-18792) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000037900
NCBI RefSeq
NM_170684
Target Region
Exon 3~10
Size of Effective Region
~4.6 kb
Detailed Document
Overview of Gene Research
Cpne7, also known as Copine7, is a gene with significant roles in various biological processes. It is involved in supporting periodontal attachment and alignment, and plays a part in the maintenance and homeostasis of odontoblasts, promoting their differentiation from progenitor cells [1,2]. It may also be associated with the amyloidogenic pathway in Alzheimer's disease by regulating ADAM10 translation [4]. Genetic models, such as KO/CKO mouse models, are valuable for studying its functions.
In Cpne7-null dental pulp, cellular senescence in odontoblasts is accelerated due to oxidative stress and DNA damage accumulation, impairing odontoblast survival and leading to aberrant dentin formation [1]. In contrast, Cpne7-overexpressing transgenic mice show a healthy dentin-pulp complex and the ability to regenerate physiologic dentin in artificial defects [1]. In colorectal cancer, Cpne7 is highly expressed and promotes cancer cell proliferation and metastasis. Knockdown of Cpne7 inhibits these processes, highlighting its oncogenic role [3,5,6].
In conclusion, Cpne7 is essential for maintaining the function of odontoblasts and periodontal tissues, and its dysregulation is associated with colorectal cancer. Gene knockout mouse models have been crucial in revealing its role in age-related dental diseases and colorectal cancer, providing insights into potential therapeutic targets for these conditions.
References:
1. Lee, Yoon Seon, Park, Yeoung-Hyun, Hwang, Geumbit, Lee, Gene, Park, Joo-Cheol. 2023. Cpne7 deficiency induces cellular senescence and premature aging of dental pulp. In Aging cell, 23, e14061. doi:10.1111/acel.14061. https://pubmed.ncbi.nlm.nih.gov/38105557/
2. Bai, Shengfeng, Lee, Ji-Hyun, Son, Chul, Lee, Dong-Seol, Park, Joo-Cheol. 2022. CPNE7 regenerates periodontal ligament via TAU-mediated alignment and cementum attachment protein-mediated attachment. In Journal of clinical periodontology, 49, 609-620. doi:10.1111/jcpe.13621. https://pubmed.ncbi.nlm.nih.gov/35373365/
3. Zhao, Liangbo, Sun, Xiao, Hou, Chenying, Jin, Shuiling, Liu, Benyu. 2024. CPNE7 promotes colorectal tumorigenesis by interacting with NONO to initiate ZFP42 transcription. In Cell death & disease, 15, 896. doi:10.1038/s41419-024-07288-z. https://pubmed.ncbi.nlm.nih.gov/39695095/
4. Yang, Jie, Pu, Ya-Lan, Pan, Qiu-Lin, Zhu, Bing-Lin, Chen, Guo-Jun. . CPNE7 Regulates Amyloidogenesis Through CAP1-Dependent ADAM10 Translation. In Journal of neurochemistry, 169, e70026. doi:10.1111/jnc.70026. https://pubmed.ncbi.nlm.nih.gov/40026213/
5. Xu, Weile, Tang, Yujie, Yang, Yang, Zhao, Lianmei, Wang, Guiying. . Depletion of CPNE7 sensitizes colorectal cancer to 5-fluorouracil by downregulating ATG9B expression. In Journal of cellular and molecular medicine, 28, e18261. doi:10.1111/jcmm.18261. https://pubmed.ncbi.nlm.nih.gov/38526029/
6. Kong, Hye-Jeong, Kang, Dong-Hyun, Ahn, Tae-Sung, Ryu, Jae-Sung, Beak, Moo-Jun. 2023. The Role of CPNE7 (Copine-7) in Colorectal Cancer Prognosis and Metastasis. In International journal of molecular sciences, 24, . doi:10.3390/ijms242316704. https://pubmed.ncbi.nlm.nih.gov/38069026/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen