C57BL/6JCya-Mpeg1em1/Cya
Common Name:
Mpeg1-KO
Product ID:
S-KO-18801
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mpeg1-KO
Strain ID
KOCMP-17476-Mpeg1-B6J-VA
Gene Name
Product ID
S-KO-18801
Gene Alias
MPS1; Mpg-1; P-2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mpeg1em1/Cya mice (Catalog S-KO-18801) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000081035
NCBI RefSeq
NM_010821
Target Region
Exon 1~2
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Mpeg1, also known as Perforin-2, is a pore-forming protein encoded by the macrophage-expressed gene 1. It belongs to the Membrane Attack Complex/Perforin (MACPF) branch of the MACPF/Cholesterol Dependent Cytolysin (CDC) superfamily. Mpeg1 is highly expressed in macrophages and other phagocytes, and is postulated to play a role in immunity, with its expression upregulated in response to pro-inflammatory signals [2].
Some studies using Mpeg1 knockout mice showed conflicting results. Initially, Mpeg1tm1Pod homozygous mutant mice were reported to succumb to bacterial infection and have deficiencies in bacterial killing in vitro [1]. However, mice homozygous for the new allele Mpeg1tm1.1Pib were not abnormally susceptible to bacterial or viral infection, and showed no perturbation in bacterial killing in vitro [1]. Other research indicated that Mpeg1 -deficient mice had increased susceptibility to microbial infection, with Mpeg1 -knockout mice suffering higher mortality rates when challenged with gram-negative or-positive pathogens [3,4]. Also, in humans, germline mutations in Mpeg1 have been associated with recurrent pulmonary mycobacterial infections and persistent polymicrobial skin and soft tissue infections [2,3]. Moreover, Mpeg1 -deficient mice failed to efficiently prime CD8+ T cells to cell-associated antigens, revealing its role in cytosolic entry of antigens during cross-presentation [5].
In conclusion, Mpeg1 is likely an intracellular pore-forming immune effector. Its role in antibacterial and antiviral immunity remains somewhat controversial, but it may play a role in antigen presentation and is clearly important in the context of certain human infections, such as pulmonary mycobacterial and skin and soft tissue infections. Studies on Mpeg1 knockout mouse models have been crucial in understanding its functions in these biological processes and disease conditions [1-2,4-5,9].
References:
1. Ebrahimnezhaddarzi, Salimeh, Bird, Catherina H, Allison, Cody C, Whisstock, James C, Bird, Phillip I. 2022. Mpeg1 is not essential for antibacterial or antiviral immunity, but is implicated in antigen presentation. In Immunology and cell biology, 100, 529-546. doi:10.1111/imcb.12554. https://pubmed.ncbi.nlm.nih.gov/35471730/
2. Bayly-Jones, Charles, Pang, Siew Siew, Spicer, Bradley A, Whisstock, James C, Dunstone, Michelle A. 2020. Ancient but Not Forgotten: New Insights Into MPEG1, a Macrophage Perforin-Like Immune Effector. In Frontiers in immunology, 11, 581906. doi:10.3389/fimmu.2020.581906. https://pubmed.ncbi.nlm.nih.gov/33178209/
3. Merselis, Leidy C, Jiang, Shirley Y, Nelson, Stanley F, Munson, George P, Butte, Manish J. 2020. MPEG1/Perforin-2 Haploinsufficiency Associated Polymicrobial Skin Infections and Considerations for Interferon-γ Therapy. In Frontiers in immunology, 11, 601584. doi:10.3389/fimmu.2020.601584. https://pubmed.ncbi.nlm.nih.gov/33224153/
4. Merselis, Leidy C, Rivas, Zachary P, Munson, George P. 2021. Breaching the Bacterial Envelope: The Pivotal Role of Perforin-2 (MPEG1) Within Phagocytes. In Frontiers in immunology, 12, 597951. doi:10.3389/fimmu.2021.597951. https://pubmed.ncbi.nlm.nih.gov/33692780/
5. Rodríguez-Silvestre, Pablo, Laub, Marco, Krawczyk, Patrycja A, Borner, Georg H H, Kozik, Patrycja. 2023. Perforin-2 is a pore-forming effector of endocytic escape in cross-presenting dendritic cells. In Science (New York, N.Y.), 380, 1258-1265. doi:10.1126/science.adg8802. https://pubmed.ncbi.nlm.nih.gov/37347855/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen