C57BL/6JCya-Zfp384em1/Cya
Common Name:
Zfp384-KO
Product ID:
S-KO-18835
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Zfp384-KO
Strain ID
KOCMP-269800-Zfp384-B6J-VA
Gene Name
Product ID
S-KO-18835
Gene Alias
C130073D16Rik; Ciz; NP; Nmp4
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp384em1/Cya mice (Catalog S-KO-18835) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000112427
NCBI RefSeq
NM_001372422
Target Region
Exon 3~6
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Zfp384, also known as CIZ/NMP4 (Cas interacting zinc finger protein, Nuclear Matrix Protein 4), is a transcription factor. It is involved in regulating matrix-related proteins, and is associated with pathways related to bone metabolism, immune response, and cell cycle regulation [1,2,3]. Genetic models, such as knockout (KO) mice, are valuable for studying its functions.
In KO mouse models, CIZ/NMP4 deficiency suppresses the development of K/BxN-serum induced arthritis, reducing the invasion of inflammatory cells, bone resorption, and cartilage matrix degradation. This is likely through its regulation of key molecules like IL-1β, RANKL, and MMP-3 [1]. Nmp4-null mice have enhanced responses to intermittent parathyroid hormone (PTH) in terms of increasing trabecular bone mass and are immune to disuse-induced bone loss, suggesting it suppresses osteoblast function [2]. Conditional loss of Nmp4 in mesenchymal stem progenitor cells (MSPCs) enhances PTH-induced bone formation and Scl-mAb-induced increases in trabecular bone, while deletion in later osteoblast stages does not replicate these responses [4,5]. Nmp4-deficient mice are also protected from H1N1 influenza infection, with reduced recruitment of monocytes and neutrophils to the lungs and decreased expression of chemokine and pro-inflammatory cytokine genes [6].
In conclusion, Zfp384 plays crucial roles in bone metabolism, arthritis development, and the innate immune response. The study of Zfp384 KO and conditional knockout (CKO) mouse models has provided valuable insights into its functions in these disease-related biological processes, potentially guiding the development of new therapeutic strategies for osteoporosis, arthritis, and influenza-related lung inflammation.
References:
1. Nakamoto, Tetsuya, Izu, Yayoi, Kawasaki, Makiri, Noda, Masaki, Ezura, Yoichi. 2015. Mice Deficient in CIZ/NMP4 Develop an Attenuated Form of K/BxN-Serum Induced Arthritis. In Journal of cellular biochemistry, 117, 970-7. doi:10.1002/jcb.25382. https://pubmed.ncbi.nlm.nih.gov/26378628/
2. Childress, Paul, Robling, Alexander G, Bidwell, Joseph P. 2009. Nmp4/CIZ: road block at the intersection of PTH and load. In Bone, 46, 259-66. doi:10.1016/j.bone.2009.09.014. https://pubmed.ncbi.nlm.nih.gov/19766748/
3. Yin, Li, Guo, Xueqiang, Zhang, Chunyan, Cai, Zhihui, Xu, Cunshuan. 2018. In silico analysis of expression data during the early priming stage of liver regeneration after partial hepatectomy in rat. In Oncotarget, 9, 11794-11804. doi:10.18632/oncotarget.24370. https://pubmed.ncbi.nlm.nih.gov/29545936/
4. Atkinson, Emily G, Adaway, Michele, Horan, Daniel J, Robling, Alexander G, Bidwell, Joseph P. 2022. Conditional Loss of Nmp4 in Mesenchymal Stem Progenitor Cells Enhances PTH-Induced Bone Formation. In Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 38, 70-85. doi:10.1002/jbmr.4732. https://pubmed.ncbi.nlm.nih.gov/36321253/
5. Korff, Crystal, Adaway, Michele, Atkinson, Emily G, Robling, Alexander G, Bidwell, Joseph P. 2023. Loss of Nmp4 enhances bone gain from sclerostin antibody administration. In Bone, 177, 116891. doi:10.1016/j.bone.2023.116891. https://pubmed.ncbi.nlm.nih.gov/37660938/
6. Yang, Shuangshuang, Adaway, Michele, Du, Jianguang, Bidwell, Joseph P, Zhou, Baohua. 2020. NMP4 regulates the innate immune response to influenza A virus infection. In Mucosal immunology, 14, 209-218. doi:10.1038/s41385-020-0280-z. https://pubmed.ncbi.nlm.nih.gov/32152414/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen