C57BL/6JCya-Blkem1/Cya
Common Name
Blk-KO
Product ID
S-KO-18856
Backgroud
C57BL/6JCya
Strain ID
KOCMP-12143-Blk-B6J-VB
When using this mouse strain in a publication, please cite “Blk-KO Mouse (Catalog S-KO-18856) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Blk-KO
Strain ID
KOCMP-12143-Blk-B6J-VB
Gene Name
Product ID
S-KO-18856
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000014597
NCBI RefSeq
NM_007549
Target Region
Exon 3~4
Size of Effective Region
~2.3 kb
Overview of Gene Research
Blk, also known as B-cell lymphocyte kinase, is a member of the SRC family nonreceptor tyrosine kinase. It is involved in the B-cell receptor (BCR) signaling pathway, playing a crucial role in B cell development and function. Dysregulation of Blk is associated with autoimmune diseases and cancer [3].
BLK-deficient mice produce less inflammatory cytokines and are more resistant to death upon IL-1β challenge, indicating that BLK positively regulates TLR/IL-1R-mediated inflammatory response [1]. BLK-deficient mice also exhibit lower serum cytokine levels and higher lethality after VSV infection, suggesting that BLK is a key kinase positively modulating IRF3-dependent signaling cascades and executing an antiviral effect [4]. In addition, in R-CHOP treated diffuse large B-cell lymphoma patients, BLK expression was associated with a poor prognosis [2].
In conclusion, Blk has essential functions in B cell-related signaling pathways and antiviral responses. The study of Blk knockout mouse models has provided insights into its role in inflammatory responses, antiviral immunity, and certain cancers, highlighting its significance in understanding disease mechanisms and potentially developing new therapeutic strategies.
References：
1. Li, Wei-Wei, Fan, Xu-Xu, Xu, Zhi-Sheng, Wang, Yan-Yi, Zheng, Hai-Xue. 2023. BLK positively regulates TLR/IL-1R signaling by catalyzing TOLLIP phosphorylation. In The Journal of cell biology, 223, . doi:10.1083/jcb.202302081. https://pubmed.ncbi.nlm.nih.gov/38078859/
2. Choi, Soyeon, Lee, Yoo Jin, Choi, Yunsuk, Cha, Hee Jeong, Jo, Jae-Cheol. 2022. Prognostic significance of BLK expression in R-CHOP treated diffuse large B-cell lymphoma. In Journal of pathology and translational medicine, 56, 281-288. doi:10.4132/jptm.2022.07.26. https://pubmed.ncbi.nlm.nih.gov/36128864/
3. Fu, Tiancheng, Zuo, Yingying, Zhong, Zhenpeng, Chen, Xuan, Pan, Zhengying. 2021. Discovery of selective irreversible inhibitors of B-Lymphoid tyrosine kinase (BLK). In European journal of medicinal chemistry, 229, 114051. doi:10.1016/j.ejmech.2021.114051. https://pubmed.ncbi.nlm.nih.gov/34952433/
4. Li, Wei-Wei, Fan, Xu-Xu, Zhu, Zi-Xiang, Wang, Yan-Yi, Zheng, Hai-Xue. 2023. Tyrosine phosphorylation of IRF3 by BLK facilitates its sufficient activation and innate antiviral response. In PLoS pathogens, 19, e1011742. doi:10.1371/journal.ppat.1011742. https://pubmed.ncbi.nlm.nih.gov/37871014/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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