C57BL/6JCya-Ilkem1/Cya
Common Name:
Ilk-KO
Product ID:
S-KO-18858
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ilk-KO
Strain ID
KOCMP-16202-Ilk-B6J-VA
Gene Name
Product ID
S-KO-18858
Gene Alias
ESTM24; ILK-1; ILK-2; p59ILK
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ilkem1/Cya mice (Catalog S-KO-18858) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033182
NCBI RefSeq
NM_001161724
Target Region
Exon 3~13
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Integrin-linked kinase (ILK) is a multifunctional protein involved in cell-matrix interactions, cell adhesion, and anchorage-dependent cell growth. It functions as a signal transducer and a scaffold protein within the ILK-PINCH-Parvin complex, and is associated with integrin and growth factor receptor signalling pathways. ILK is crucial for cellular processes like proliferation, survival, differentiation, migration, invasion, and angiogenesis, and its dysfunction is linked to various diseases including cancer [1,3,4].
In endothelial cell-specific ILK conditional knock-out (ecILK cKO) mice, endothelial ILK deletion significantly impaired cardiac function, leading to systolic and diastolic dysfunction, extensive extracellular matrix remodelling, perivascular fibrosis, and early ischaemic-like events. The mice also exhibited features of coronary microvascular disease, and endothelial cells underwent endothelial-to-mesenchymal transition (endMT), indicating that endothelial ILK is a crucial regulator of microvascular endothelial homeostasis [2]. In cisplatin-resistant ovarian cancer cells, targeting ILK by small-interfering RNA reduced cell growth and invasion, and increased apoptosis. Phosphorylated ILK levels were higher in cisplatin-resistant ovarian cancer cells and tumor tissue compared to sensitive cells and normal ovarian epithelium [5].
In conclusion, ILK is essential for maintaining normal physiological functions in various tissues, especially in microvascular endothelial homeostasis. Studies using ILK KO/CKO mouse models have revealed its significant role in cardiac function and microvascular diseases, as well as in cancer development. Understanding ILK's functions can potentially provide new therapeutic strategies for related diseases.
References:
1. Górska, Agata, Mazur, Antonina Joanna. 2022. Integrin-linked kinase (ILK): the known vs. the unknown and perspectives. In Cellular and molecular life sciences : CMLS, 79, 100. doi:10.1007/s00018-021-04104-1. https://pubmed.ncbi.nlm.nih.gov/35089438/
2. Reventun, P, Sánchez-Esteban, S, Cook-Calvete, A, Zaragoza, C, Saura, M. 2023. Endothelial ILK induces cardioprotection by preventing coronary microvascular dysfunction and endothelial-to-mesenchymal transition. In Basic research in cardiology, 118, 28. doi:10.1007/s00395-023-00997-0. https://pubmed.ncbi.nlm.nih.gov/37452166/
3. Qin, Jun, Wu, Chuanyue. 2012. ILK: a pseudokinase in the center stage of cell-matrix adhesion and signaling. In Current opinion in cell biology, 24, 607-13. doi:10.1016/j.ceb.2012.06.003. https://pubmed.ncbi.nlm.nih.gov/22763012/
4. Zheng, Can-Can, Hu, Hui-Fang, Hong, Pan, He, Qing-Yu, Li, Bin. 2019. Significance of integrin-linked kinase (ILK) in tumorigenesis and its potential implication as a biomarker and therapeutic target for human cancer. In American journal of cancer research, 9, 186-197. doi:. https://pubmed.ncbi.nlm.nih.gov/30755822/
5. Reyes-González, Jeyshka M, Quiñones-Díaz, Blanca I, Santana, Yasmarie, Fernández-de Thomas, Ricardo J, Vivas-Mejía, Pablo E. 2020. Downstream Effectors of ILK in Cisplatin-Resistant Ovarian Cancer. In Cancers, 12, . doi:10.3390/cancers12040880. https://pubmed.ncbi.nlm.nih.gov/32260415/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen