Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Mir125b-1em1/Cya
Common Name:
Mir125b-1-KO
Product ID:
S-KO-18879
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mir125b-1-KO
Strain ID
KOCMP-387236-Mir125b-1-B6J-VA
Gene Name
Mir125b-1
Product ID
S-KO-18879
Gene Alias
Mirn125b; Mirn125b-1; mir-125b-1
Background
C57BL/6JCya
NCBI ID
387236
Modification
Conventional knockout
Chromosome
9
Phenotype
MGI:2676810
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mir125b-1em1/Cya mice (Catalog S-KO-18879) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000175613
NCBI RefSeq
NR_029822
Target Region
Exon 1
Size of Effective Region
~0.1 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Mir125b-1 is a microRNA-encoding gene. MicroRNAs are crucial regulators in various biological processes, often involved in post-transcriptional regulation of gene expression by binding to target mRNAs [1-8].

In human brain, Mir125b-1 is not imprinted, which is different from its isoform Mir125b-2. In mouse brain, it is highly expressed but down-regulated during development, with preferential expression in the olfactory bulb, thalamus, and hypothalamus, and is enriched in GABAergic neurons, especially somatostatin-expressing ones [1].

In metastatic clear cell renal cell carcinoma, high methylation levels of Mir125b-1 are correlated with late stages, large tumor size, metastasis, and loss of differentiation [2]. In breast cancer, increased methylation of Mir125b-1 in tumors compared to normal tissues was observed, and it was part of an optimized marker system for early diagnosis. Detection of its methylation was sufficient to classify samples as breast cancer [3].

In melanogenesis, reduction of miR-125b expression in pigmented cells was due to hypermethylation of the Mir125b-1 promoter [4]. In non-small cell lung cancer, a significantly high level of Mir125b-1 methylation was found in adenocarcinoma and squamous cell lung cancer compared to adjacent normal tissue [5].

In kidney cancer, increased methylation of Mir125b-1 was associated with distant metastases, and a combined panel including this gene could estimate metastasis probability with high accuracy [6]. In ovarian carcinoma, Mir125b-1 was hypermethylated at early stages, and in breast cancer, its hypermethylation was associated with lymph node metastasis and a system for predicting metastasis was developed based on it [7,8].

In conclusion, Mir125b-1 is involved in multiple biological and disease-related processes. Its non-imprinting in the human brain and specific spatiotemporal expression in the mouse brain contribute to understanding brain development. In various cancers such as renal, breast, lung, and ovarian cancers, changes in its methylation status are associated with tumor progression, metastasis, and can be used as potential biomarkers, highlighting the importance of studying Mir125b-1 in disease diagnosis and prognosis.

References:
1. Hou, Kuan-Chu, Tsai, Meng-Han, Akbarian, Schahram, Huang, Hsien-Sung. 2023. Mir125b-1 is Not Imprinted in Human Brain and Shows Developmental Expression Changes in Mouse Brain. In Neuroscience, 529, 99-106. doi:10.1016/j.neuroscience.2023.08.014. https://pubmed.ncbi.nlm.nih.gov/37598835/
2. Ivanova, N A, Burdennyi, A M, Lukina, S S, Braga, E A, Kushlinskii, N E. 2023. The Role of Methylation of a Group of microRNA Genes in the Pathogenesis of Metastatic Renal Cell Carcinoma. In Bulletin of experimental biology and medicine, 175, 249-253. doi:10.1007/s10517-023-05844-9. https://pubmed.ncbi.nlm.nih.gov/37466853/
3. Burdennyy, A M, Filippova, E A, Khodyrev, D S, Loginov, V I, Braga, E A. 2021. Optimized Marker System for Early Diagnosis of Breast Cancer. In Bulletin of experimental biology and medicine, 172, 57-62. doi:10.1007/s10517-021-05331-z. https://pubmed.ncbi.nlm.nih.gov/34791555/
4. Kim, Kyu-Han, Bin, Bum-Ho, Kim, Juewon, Cho, Eun-Gyung, Lee, Tae Ryong. 2013. Novel inhibitory function of miR-125b in melanogenesis. In Pigment cell & melanoma research, 27, 140-4. doi:10.1111/pcmr.12179. https://pubmed.ncbi.nlm.nih.gov/24118912/
5. Gubenko, M S, Loginov, V I, Burdennyy, A M, Kazubskaya, T P, Pertsov, S S. 2023. Changes in the Level of Methylation of a Group of microRNA Genes as a Factor in the Development and Progression of Non-Small Cell Lung Cancer. In Bulletin of experimental biology and medicine, 174, 254-258. doi:10.1007/s10517-023-05684-7. https://pubmed.ncbi.nlm.nih.gov/36598670/
6. Apanovich, Natalya, Matveev, Alexey, Ivanova, Natalia, Braga, Eleonora, Alimov, Andrei. 2023. Prediction of Distant Metastases in Patients with Kidney Cancer Based on Gene Expression and Methylation Analysis. In Diagnostics (Basel, Switzerland), 13, . doi:10.3390/diagnostics13132289. https://pubmed.ncbi.nlm.nih.gov/37443682/
7. Loginov, Vitaly I, Pronina, Irina V, Filippova, Elena A, Dmitriev, Alexey A, Braga, Eleonora A. 2022. Aberrant Methylation of 20 miRNA Genes Specifically Involved in Various Steps of Ovarian Carcinoma Spread: From Primary Tumors to Peritoneal Macroscopic Metastases. In International journal of molecular sciences, 23, . doi:10.3390/ijms23031300. https://pubmed.ncbi.nlm.nih.gov/35163224/
8. Loginov, V I, Burdennyy, A M, Filippova, E A, Khodyrev, D S, Braga, E A. 2021. Aberrant Methylation of 21 MicroRNA Genes in Breast Cancer: Sets of Genes Associated with Progression and a System of Markers for Predicting Metastasis. In Bulletin of experimental biology and medicine, 172, 67-71. doi:10.1007/s10517-021-05333-x. https://pubmed.ncbi.nlm.nih.gov/34792716/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest