C57BL/6JCya-Asf1aem1/Cya
Common Name:
Asf1a-KO
Product ID:
S-KO-18882
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Asf1a-KO
Strain ID
KOCMP-66403-Asf1a-B6J-VA
Gene Name
Product ID
S-KO-18882
Gene Alias
2310079C17Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Asf1aem1/Cya mice (Catalog S-KO-18882) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020004
NCBI RefSeq
NM_025541
Target Region
Exon 2
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
Asf1a, short for anti-silencing function 1a, is a histone H3-H4 chaperone isoform. It is involved in chromatin assembling, transcription regulation, and various chromatin-mediated cellular processes [3,5]. It participates in pathways related to cell differentiation, DNA repair, and immunity, holding great biological importance. Genetic models, such as KO/CKO mouse models, are valuable for studying its functions.
In atherosclerosis, endothelium-specific Asf1a deficiency in Apoe KO Asf1a ECKO mice inhibits endothelial-to-mesenchymal transition (EndMT) and alleviates atherosclerosis development. This indicates that Asf1a, as a cofactor of P300, promotes atherosclerosis by regulating EndMT through histone H3 lysine 18 lactylation [1]. In Kras-mutant lung adenocarcinoma, loss of Asf1a in tumor cells in a mouse model sensitizes tumors to anti-PD-1 treatment, suggesting its potential as an immunotherapeutic target [2]. In cancer cells, ASF1a knockdown leads to growth arrest and senescence in wild-type p53-carrying cells, revealing its importance in cancer cell proliferation [3]. In chronic myeloid leukemia, ASF1A is up-regulated in blast crisis patients, and its inhibition may be a therapeutic approach as it activates Notch signaling to mediate differentiation arrest [4].
In conclusion, Asf1a is crucial in multiple biological processes. Model-based research, especially KO/CKO mouse models, has revealed its roles in diseases like atherosclerosis, lung adenocarcinoma, various cancers, and chronic myeloid leukemia. These findings provide potential therapeutic targets and biomarkers for these disease areas.
References:
1. Dong, Mengdie, Zhang, Yunjia, Chen, Minghong, Ji, Yong, Chen, Hongshan. 2024. ASF1A-dependent P300-mediated histone H3 lysine 18 lactylation promotes atherosclerosis by regulating EndMT. In Acta pharmaceutica Sinica. B, 14, 3027-3048. doi:10.1016/j.apsb.2024.03.008. https://pubmed.ncbi.nlm.nih.gov/39027248/
2. Li, Fei, Huang, Qingyuan, Luster, Troy A, Miller, George, Wong, Kwok-Kin. 2019. In Vivo Epigenetic CRISPR Screen Identifies Asf1a as an Immunotherapeutic Target in Kras-Mutant Lung Adenocarcinoma. In Cancer discovery, 10, 270-287. doi:10.1158/2159-8290.CD-19-0780. https://pubmed.ncbi.nlm.nih.gov/31744829/
3. Wu, Yujiao, Li, Xidan, Yu, Jingya, Björkholm, Magnus, Xu, Dawei. 2019. ASF1a inhibition induces p53-dependent growth arrest and senescence of cancer cells. In Cell death & disease, 10, 76. doi:10.1038/s41419-019-1357-z. https://pubmed.ncbi.nlm.nih.gov/30692519/
4. Yin, Xiaolin, Zhou, Minran, Zhang, Lu, Ma, Sai, Chen, Chunyan. 2022. Histone chaperone ASF1A accelerates chronic myeloid leukemia blast crisis by activating Notch signaling. In Cell death & disease, 13, 842. doi:10.1038/s41419-022-05234-5. https://pubmed.ncbi.nlm.nih.gov/36184659/
5. Liu, Zhaolong, Yang, Le, Sun, Yanxiang, Xie, Xiaofeng, Huang, Jianping. 2016. ASF1a enhances antiviral immune response by associating with CBP to mediate acetylation of H3K56 at the Ifnb promoter. In Molecular immunology, 78, 57-64. doi:10.1016/j.molimm.2016.08.008. https://pubmed.ncbi.nlm.nih.gov/27596240/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen