C57BL/6JCya-Aoc3em1/Cya
Common Name:
Aoc3-KO
Product ID:
S-KO-18897
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Aoc3-KO
Strain ID
KOCMP-11754-Aoc3-B6J-VB
Gene Name
Product ID
S-KO-18897
Gene Alias
SSAO; VAP1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Aoc3em1/Cya mice (Catalog S-KO-18897) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000103105
NCBI RefSeq
NM_009675
Target Region
Exon 1
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Aoc3, also known as semicarbazide-sensitive amine oxidase and vascular adhesion protein 1 (VAP1), is a membrane-bound adhesion protein. It facilitates leukocyte-endothelial cell adhesion, and its enzymatic function involves catalyzing the oxidative deamination of primary amines to generate hydrogen peroxide (H2O2). It is associated with inflammation-related pathways and plays important roles in various biological processes and diseases [1,4].
Aoc3 deficiency in mice has shown several impacts. In ApoE-/-Aoc3-/-mice, atherosclerotic plaques increased at an early stage, related to VSMC dedifferentiation and higher T-cell recruitment in plaques due to MCP-1 augmentation [2]. Aoc3 knockout mice were more prone to DSS-induced colitis and colonic tumorigenesis, with shorter survival in colitis models and more tumors in both AOM/DSS and Apc mutant mice models [3]. In the context of myocardial infarction, Aoc3 knockdown alleviated cardiac fibrosis and improved cardiac function and hypertrophy in mice [4].
In conclusion, Aoc3 is crucial in processes like inflammation, leukocyte adhesion, and oxidative stress generation. Gene knockout mouse models have revealed its role in diseases such as atherosclerosis, colitis, colonic tumorigenesis, and cardiac remodelling after myocardial infarction, providing insights into potential therapeutic targets for these diseases.
References:
1. Boyer, David S, Rippmann, Joerg F, Ehrlich, Michael S, Chong, Victor, Nguyen, Quan Dong. 2021. Amine oxidase copper-containing 3 (AOC3) inhibition: a potential novel target for the management of diabetic retinopathy. In International journal of retina and vitreous, 7, 30. doi:10.1186/s40942-021-00288-7. https://pubmed.ncbi.nlm.nih.gov/33845913/
2. Filip, Anna, Taleb, Soraya, Bascetin, Rümeyza, Jalkanen, Sirpa, Mercier, Nathalie. 2022. Increased atherosclerotic plaque in AOC3 knock-out in ApoE-/- mice and characterization of AOC3 in atherosclerotic human coronary arteries. In Frontiers in cardiovascular medicine, 9, 848680. doi:10.3389/fcvm.2022.848680. https://pubmed.ncbi.nlm.nih.gov/36176983/
3. Özcan, Özge, Akyol, Özge, Akyol, Aytekin. . Amine Oxidase, Copper Containing 3 (Aoc3) Knockout Mice Are More Prone to DSS-induced Colitis and Colonic Tumorigenesis. In In vivo (Athens, Greece), 38, 2300-2309. doi:10.21873/invivo.13695. https://pubmed.ncbi.nlm.nih.gov/39187313/
4. Zhong, Chongbin, Que, Dongdong, Yu, Wenjie, Yan, Jing, Yang, Pingzhen. 2023. Amine oxidase copper-containing 3 aggravates cardiac remodelling by generating hydrogen peroxide after myocardial infarction. In The Journal of pathology, 260, 190-202. doi:10.1002/path.6075. https://pubmed.ncbi.nlm.nih.gov/36825552/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen