C57BL/6JCya-Upp1em1/Cya
Common Name:
Upp1-KO
Product ID:
S-KO-18902
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Upp1-KO
Strain ID
KOCMP-22271-Upp1-B6J-VB
Gene Name
Product ID
S-KO-18902
Gene Alias
UPase; UdRPase; Up; Upp
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Upp1em1/Cya mice (Catalog S-KO-18902) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020677
NCBI RefSeq
NM_009477
Target Region
Exon 6
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
UPP1, short for Uridine phosphorylase 1, is a dimeric enzyme crucial for pyrimidine salvage and uridine homeostasis. It catalyzes the reversible phosphorylation of uridine to uracil and ribose-1-phosphate [2,3]. UPP1-related pathways include the salvage of ribose from uridine, which can support glycolysis in nutrient-limited conditions, entering downstream of highly regulated steps of glucose transport and upper glycolysis [6].
In various cancers, UPP1 has been shown to play oncogenic roles. In lung adenocarcinoma (LUAD), UPP1high tumor cells are associated with an immunosuppressive tumor microenvironment. UPP1 upregulation leads to increased release of immunosuppressive cytokines like TGF-β1 and elevation of PD-L1 expression via the PI3K/AKT/mTOR pathway, suppressing CD8+ T cells [1]. UPP1 also promotes LUAD progression by driving glycolytic metabolism, and is epigenetically regulated through histone acetylation [2]. In bladder cancer, UPP1 promotes cell proliferation, migration, invasion, and gemcitabine resistance by activating the AKT signaling pathway [3]. In gliomas, high levels of UPP1 are linked to poor survival, and it promotes tumor cell proliferation, invasion, and immune evasion [5]. In pancreatic ductal adenocarcinoma, UPP1 is regulated by KRAS-MAPK signalling, and its deletion restricts tumor growth in immunocompetent mouse models as it liberates uridine-derived ribose to support cancer cell survival and proliferation in glucose-restricted conditions [4].
In conclusion, UPP1 is a key enzyme in pyrimidine metabolism and is significantly involved in the progression of multiple cancers, including LUAD, bladder cancer, gliomas, and pancreatic cancer. Functional studies, especially those using gene knockout or conditional knockout mouse models, have revealed its role in promoting tumorigenesis, immunosuppression, and metabolic reprogramming in these disease conditions, highlighting its potential as a therapeutic target [1,2,3,4,5].
References:
1. Li, Yin, Jiang, Manling, Aye, Ling, Ge, Di, Lu, Chunlai. 2024. UPP1 promotes lung adenocarcinoma progression through the induction of an immunosuppressive microenvironment. In Nature communications, 15, 1200. doi:10.1038/s41467-024-45340-w. https://pubmed.ncbi.nlm.nih.gov/38331898/
2. Wang, Xuan, Wang, Zheng, Huang, Renhong, Chen, Xiaofeng, Huang, Dayu. 2022. UPP1 Promotes Lung Adenocarcinoma Progression through Epigenetic Regulation of Glycolysis. In Aging and disease, 13, 1488-1503. doi:10.14336/AD.2022.0218. https://pubmed.ncbi.nlm.nih.gov/36186123/
3. Du, Wenzhi, Tu, Sheng, Zhang, Wenxiu, Wang, Xinghuan, Ju, Lingao. 2024. UPP1 enhances bladder cancer progression and gemcitabine resistance through AKT. In International journal of biological sciences, 20, 1389-1409. doi:10.7150/ijbs.83774. https://pubmed.ncbi.nlm.nih.gov/38385072/
4. Nwosu, Zeribe C, Ward, Matthew H, Sajjakulnukit, Peter, Sadanandam, Anguraj, Lyssiotis, Costas A. 2023. Uridine-derived ribose fuels glucose-restricted pancreatic cancer. In Nature, 618, 151-158. doi:10.1038/s41586-023-06073-w. https://pubmed.ncbi.nlm.nih.gov/37198494/
5. Chen, Zigui, Liu, Chao, Zhang, Chunyuan, Miao, Changfeng, Luo, Qisheng. 2024. Machine learning-based discovery of UPP1 as a key oncogene in tumorigenesis and immune escape in gliomas. In Frontiers in immunology, 15, 1475206. doi:10.3389/fimmu.2024.1475206. https://pubmed.ncbi.nlm.nih.gov/39380997/
6. Skinner, Owen S, Blanco-Fernández, Joan, Goodman, Russell P, Mootha, Vamsi K, Jourdain, Alexis A. 2023. Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions. In Nature metabolism, 5, 765-776. doi:10.1038/s42255-023-00774-2. https://pubmed.ncbi.nlm.nih.gov/37198474/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen