C57BL/6JCya-Dsg3em1/Cya
Common Name:
Dsg3-KO
Product ID:
S-KO-18908
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dsg3-KO
Strain ID
KOCMP-13512-Dsg3-B6J-VB
Gene Name
Product ID
S-KO-18908
Gene Alias
bal
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
18
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dsg3em1/Cya mice (Catalog S-KO-18908) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000070892
NCBI RefSeq
NM_030596
Target Region
Exon 2~5
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Dsg3, also known as DG3, is a transmembrane glycoprotein primarily found in desmosomes of keratinocytes in squamous epithelium. It mediates cell adhesion within desmosomes, playing a crucial role in maintaining the integrity of the skin and mucosa [2,3]. Dysregulation of Dsg3-related processes can lead to various diseases, highlighting its biological importance.
In pemphigus vulgaris, an autoimmune blistering disease, autoantibodies against Dsg3 disrupt desmosomes and cellular cohesion. Different epitope-specific autoantibodies have varying pathogenic effects. For example, the murine IgG AK23, directed against the EC1 domain of Dsg3, is more effective in causing loss of cell adhesion and induces Dsg3 depletion, phosphorylation of Src, p38MAPK, and Akt, while 2G4 (against EC5 domain) has milder effects [1]. The diversity of serum anti-DSG3 IgG subclasses impacts pemphigus activity and can predict relapses after treatment with rituximab [4]. In cancer, Dsg3 shows differential expression. It is overexpressed in head and neck cancer, and its inhibition reduces cell growth, colony formation, migration, and invasion in vitro and tumor growth in vivo, suggesting it as a potential therapeutic target [5]. In rectal adenocarcinoma, DSG3 expression negatively correlates with tumor regression and has an independent negative impact on survival, serving as a prognostic factor and predictor for neoadjuvant concurrent chemoradiotherapy response [6].
In conclusion, Dsg3 is essential for maintaining the integrity of the skin and mucosa through its role in cell adhesion. Studies on Dsg3 in disease models, especially in pemphigus and cancer, have provided insights into its functions and potential as a therapeutic target. Understanding Dsg3-related mechanisms in these diseases may offer new strategies for treatment.
References:
1. Schmitt, Thomas, Hudemann, Christoph, Moztarzadeh, Sina, Tikkanen, Ritva, Waschke, Jens. 2023. Dsg3 epitope-specific signalling in pemphigus. In Frontiers in immunology, 14, 1163066. doi:10.3389/fimmu.2023.1163066. https://pubmed.ncbi.nlm.nih.gov/37143675/
2. Schmidt, Enno, Kasperkiewicz, Michael, Joly, Pascal. . Pemphigus. In Lancet (London, England), 394, 882-894. doi:10.1016/S0140-6736(19)31778-7. https://pubmed.ncbi.nlm.nih.gov/31498102/
3. Fuchs, Michael, Radeva, Mariya Y, Spindler, Volker, Kugelmann, Daniela, Waschke, Jens. 2023. Cytoskeletal anchorage of different Dsg3 pools revealed by combination of hybrid STED/SMFS-AFM. In Cellular and molecular life sciences : CMLS, 80, 25. doi:10.1007/s00018-022-04681-9. https://pubmed.ncbi.nlm.nih.gov/36602635/
4. Golinski, Marie-Laure, Lemieux, Alexandre, Maho-Vaillant, Maud, Joly, Pascal, Hébert, Vivien. 2022. The Diversity of Serum Anti-DSG3 IgG Subclasses Has a Major Impact on Pemphigus Activity and Is Predictive of Relapses After Treatment With Rituximab. In Frontiers in immunology, 13, 849790. doi:10.3389/fimmu.2022.849790. https://pubmed.ncbi.nlm.nih.gov/35371083/
5. Chen, Y-J, Chang, J T, Lee, L, Chen, P-J, Cheng, A-J. 2006. DSG3 is overexpressed in head neck cancer and is a potential molecular target for inhibition of oncogenesis. In Oncogene, 26, 467-76. doi:. https://pubmed.ncbi.nlm.nih.gov/16878157/
6. Chao, Tung-Bo, Li, Chien-Feng, Lin, Ching-Yih, Chan, Ti-Chun, He, Hong-Lin. 2016. Prognostic significance of DSG3 in rectal adenocarcinoma treated with preoperative chemoradiotherapy. In Future oncology (London, England), 12, 1457-67. doi:10.2217/fon-2016-0071. https://pubmed.ncbi.nlm.nih.gov/27040321/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen