C57BL/6JCya-Adcy10em1/Cya
Common Name:
Adcy10-KO
Product ID:
S-KO-18917
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Adcy10-KO
Strain ID
KOCMP-271639-Adcy10-B6J-VA
Gene Name
Product ID
S-KO-18917
Gene Alias
4930431D04Rik; 4931412F17; Sacy; sAC
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Adcy10em1/Cya mice (Catalog S-KO-18917) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027852
NCBI RefSeq
NM_173029
Target Region
Exon 6
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Adcy10, also known as soluble adenylyl cyclase (sAC), is an enzyme involved in intracellular signaling. It is crucial for generating 3'⁻5'-cyclic adenosine monophosphate (cAMP), and its intracellular localization allows for the formation of unique cAMP microdomains controlling various physiological and pathological processes [2]. It is associated with pathways like the ADCY/PKA/HBP/YAP axis [1], and plays a significant role in multiple biological functions, such as sperm motility regulation, kidney stone-related processes, and brown adipose tissue thermogenesis.
In lung adenocarcinoma, endogenous glutamate accumulation can determine ferroptosis sensitivity by suppressing YAP via Adcy10-dependent mechanisms. Adcy10 acts as a key downstream target and diversifies the effects of glutamate on the PKA-dependent suppression of GFPT1 [1]. In the context of male fertility, proper Adcy10 (sAC) activity and a constant supply of cAMP are crucial for sperm motility regulation. A 2-bp deletion in Adcy10 coding region led to severe recessive asthenozoospermia in patients, and treatment with a cAMP analogue increased sperm motility, indicating the pathogenic role of the variant [3]. Also, in brown adipose tissue, Adcy10, as a CO2/bicarbonate sensor, mediates the effect of IDH on complex I activity through cAMP-EPAC1 signaling, maintaining the mitochondrial membrane potential and enabling thermogenesis [4].
In conclusion, Adcy10 is essential for various biological functions including sperm motility, ferroptosis sensitivity in lung adenocarcinoma, and brown adipose tissue thermogenesis. Gene-knockout or related functional studies in models like mice have been valuable in revealing its role in these processes, which has implications for understanding and potentially treating diseases such as asthenozoospermia and advanced-stage lung adenocarcinoma [1,3,4].
References:
1. Zhang, Xiao, Yu, Keke, Ma, Lifang, Yu, Yongchun, Wang, Jiayi. 2021. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. In Theranostics, 11, 5650-5674. doi:10.7150/thno.55482. https://pubmed.ncbi.nlm.nih.gov/33897873/
2. Pozdniakova, Sofya, Ladilov, Yury. 2018. Functional Significance of the Adcy10-Dependent Intracellular cAMP Compartments. In Journal of cardiovascular development and disease, 5, . doi:10.3390/jcdd5020029. https://pubmed.ncbi.nlm.nih.gov/29751653/
3. Akbari, Arvand, Pipitone, Giovanni Battista, Anvar, Zahra, Carrera, Paola, Totonchi, Mehdi. . ADCY10 frameshift variant leading to severe recessive asthenozoospermia and segregating with absorptive hypercalciuria. In Human reproduction (Oxford, England), 34, 1155-1164. doi:10.1093/humrep/dez048. https://pubmed.ncbi.nlm.nih.gov/31119281/
4. Das, Anupam, Mund, Christine, Hagag, Eman, Noll, Thomas, Alexaki, Vasileia Ismini. 2025. Adenylate cyclase 10 promotes brown adipose tissue thermogenesis. In iScience, 28, 111833. doi:10.1016/j.isci.2025.111833. https://pubmed.ncbi.nlm.nih.gov/39949963/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen