Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Myl4em1/Cya
Common Name:
Myl4-KO
Product ID:
S-KO-18932
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Myl4-KO
Strain ID
KOCMP-17896-Myl4-B6J-VB
Gene Name
Myl4
Product ID
S-KO-18932
Gene Alias
ALC1; AMLC; ELC; ELC1a; GT1; MLC1EMB; MLC1a; Myla
Background
C57BL/6JCya
NCBI ID
17896
Modification
Conventional knockout
Chromosome
11
Phenotype
MGI:97267
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Myl4em1/Cya mice (Catalog S-KO-18932) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000106957
NCBI RefSeq
NM_001355754
Target Region
Exon 4~6
Size of Effective Region
~2.9 kb
Detailed Document
Click here to download >>
Overview of Gene Research
MYL4, also known as myosin light chain 4, is crucial for muscle-related functions. It is involved in atrial development, atrial cardiomyopathy, determining muscle-fiber size, and muscle development processes like myoblast proliferation, migration, differentiation, and fusion [1,4,5,6]. It may also play a role in autophagic flux regulation in atrial cardiomyocytes [5]. Genetic models such as pigs and mice are valuable for studying MYL4.

In pigs, the SV of MYL4 was identified, and its genotype distribution differed between Ningxiang pigs and Large White pigs. Functional studies showed that overexpression of MYL4 in myoblasts inhibited proliferation and promoted apoptosis and differentiation, while knockdown had the opposite effect [1]. In Duchenne muscular dystrophy mouse models, increased Myl4 expression was observed in areas of muscle regeneration [2]. In a study on microplastics-induced cardiotoxicity in mice and human-originated cardiac organoids, the expression of cardiac-specific marker MYL4 was elevated [3]. In a zebrafish Myl4 knockout model, molecular, cellular, and physiologic abnormalities were found, paralleling those in humans with MYL4 mutations related to atrial fibrillation [7].

In conclusion, MYL4 is essential for muscle development and function, influencing myoblast behaviors, muscle fiber size, and having implications in muscle regeneration, cardiotoxicity, and atrial fibrillation. The use of gene knockout models in zebrafish and studies in other species like pigs and mice have provided insights into its role in these biological processes and disease conditions.

References:
1. Xu, Xueli, Yu, Zonggang, Ai, Nini, Ma, Haiming, Yin, Yulong. 2023. Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs. In Genes, 14, . doi:10.3390/genes14061267. https://pubmed.ncbi.nlm.nih.gov/37372447/
2. Heezen, L G M, Abdelaal, T, van Putten, M, Mahfouz, A, Spitali, P. 2023. Spatial transcriptomics reveal markers of histopathological changes in Duchenne muscular dystrophy mouse models. In Nature communications, 14, 4909. doi:10.1038/s41467-023-40555-9. https://pubmed.ncbi.nlm.nih.gov/37582915/
3. Zhou, Yue, Wu, Qian, Li, Yan, Wang, Yan, Cheng, Wei. 2023. Low-dose of polystyrene microplastics induce cardiotoxicity in mice and human-originated cardiac organoids. In Environment international, 179, 108171. doi:10.1016/j.envint.2023.108171. https://pubmed.ncbi.nlm.nih.gov/37669592/
4. Dong, Shixiong, Han, Yuqing, Zhang, Jian, Chamba, Yangzom, Shang, Peng. 2022. Haplotypes within the regulatory region of MYL4 are associated with pig muscle fiber size. In Gene, 850, 146934. doi:10.1016/j.gene.2022.146934. https://pubmed.ncbi.nlm.nih.gov/36202278/
5. Zhong, Yuan, Tang, Kai, Nattel, Stanley, Peng, Wenhui, Li, Hailing. 2023. Myosin light-chain 4 gene-transfer attenuates atrial fibrosis while correcting autophagic flux dysregulation. In Redox biology, 60, 102606. doi:10.1016/j.redox.2023.102606. https://pubmed.ncbi.nlm.nih.gov/36645977/
6. Ye, Yourong, Wu, Guoxin, Wang, Haoqi, Shang, Peng, Chamba, Yangzom. 2024. The Role of the MYL4 Gene in Porcine Muscle Development and Its Molecular Regulatory Mechanisms. In Animals : an open access journal from MDPI, 14, . doi:10.3390/ani14091370. https://pubmed.ncbi.nlm.nih.gov/38731374/
7. Ghazizadeh, Zaniar, Kiviniemi, Tuomas, Olafsson, Sigurast, Hollmén, Maija, MacRae, Calum A. 2019. Metastable Atrial State Underlies the Primary Genetic Substrate for MYL4 Mutation-Associated Atrial Fibrillation. In Circulation, 141, 301-312. doi:10.1161/CIRCULATIONAHA.119.044268. https://pubmed.ncbi.nlm.nih.gov/31735076/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest