C57BL/6JCya-Myl4em1/Cya
Common Name:
Myl4-KO
Product ID:
S-KO-18932
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Myl4-KO
Strain ID
KOCMP-17896-Myl4-B6J-VB
Gene Name
Product ID
S-KO-18932
Gene Alias
ALC1; AMLC; ELC; ELC1a; GT1; MLC1EMB; MLC1a; Myla
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Myl4em1/Cya mice (Catalog S-KO-18932) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000106957
NCBI RefSeq
NM_001355754
Target Region
Exon 4~6
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
MYL4, also known as myosin light chain 4, is crucial for muscle-related functions. It is involved in atrial development, atrial cardiomyopathy, determining muscle-fiber size, and muscle development processes like myoblast proliferation, migration, differentiation, and fusion [1,4,5,6]. It may also play a role in autophagic flux regulation in atrial cardiomyocytes [5]. Genetic models such as pigs and mice are valuable for studying MYL4.
In pigs, the SV of MYL4 was identified, and its genotype distribution differed between Ningxiang pigs and Large White pigs. Functional studies showed that overexpression of MYL4 in myoblasts inhibited proliferation and promoted apoptosis and differentiation, while knockdown had the opposite effect [1]. In Duchenne muscular dystrophy mouse models, increased Myl4 expression was observed in areas of muscle regeneration [2]. In a study on microplastics-induced cardiotoxicity in mice and human-originated cardiac organoids, the expression of cardiac-specific marker MYL4 was elevated [3]. In a zebrafish Myl4 knockout model, molecular, cellular, and physiologic abnormalities were found, paralleling those in humans with MYL4 mutations related to atrial fibrillation [7].
In conclusion, MYL4 is essential for muscle development and function, influencing myoblast behaviors, muscle fiber size, and having implications in muscle regeneration, cardiotoxicity, and atrial fibrillation. The use of gene knockout models in zebrafish and studies in other species like pigs and mice have provided insights into its role in these biological processes and disease conditions.
References:
1. Xu, Xueli, Yu, Zonggang, Ai, Nini, Ma, Haiming, Yin, Yulong. 2023. Molecular Mechanism of MYL4 Regulation of Skeletal Muscle Development in Pigs. In Genes, 14, . doi:10.3390/genes14061267. https://pubmed.ncbi.nlm.nih.gov/37372447/
2. Heezen, L G M, Abdelaal, T, van Putten, M, Mahfouz, A, Spitali, P. 2023. Spatial transcriptomics reveal markers of histopathological changes in Duchenne muscular dystrophy mouse models. In Nature communications, 14, 4909. doi:10.1038/s41467-023-40555-9. https://pubmed.ncbi.nlm.nih.gov/37582915/
3. Zhou, Yue, Wu, Qian, Li, Yan, Wang, Yan, Cheng, Wei. 2023. Low-dose of polystyrene microplastics induce cardiotoxicity in mice and human-originated cardiac organoids. In Environment international, 179, 108171. doi:10.1016/j.envint.2023.108171. https://pubmed.ncbi.nlm.nih.gov/37669592/
4. Dong, Shixiong, Han, Yuqing, Zhang, Jian, Chamba, Yangzom, Shang, Peng. 2022. Haplotypes within the regulatory region of MYL4 are associated with pig muscle fiber size. In Gene, 850, 146934. doi:10.1016/j.gene.2022.146934. https://pubmed.ncbi.nlm.nih.gov/36202278/
5. Zhong, Yuan, Tang, Kai, Nattel, Stanley, Peng, Wenhui, Li, Hailing. 2023. Myosin light-chain 4 gene-transfer attenuates atrial fibrosis while correcting autophagic flux dysregulation. In Redox biology, 60, 102606. doi:10.1016/j.redox.2023.102606. https://pubmed.ncbi.nlm.nih.gov/36645977/
6. Ye, Yourong, Wu, Guoxin, Wang, Haoqi, Shang, Peng, Chamba, Yangzom. 2024. The Role of the MYL4 Gene in Porcine Muscle Development and Its Molecular Regulatory Mechanisms. In Animals : an open access journal from MDPI, 14, . doi:10.3390/ani14091370. https://pubmed.ncbi.nlm.nih.gov/38731374/
7. Ghazizadeh, Zaniar, Kiviniemi, Tuomas, Olafsson, Sigurast, Hollmén, Maija, MacRae, Calum A. 2019. Metastable Atrial State Underlies the Primary Genetic Substrate for MYL4 Mutation-Associated Atrial Fibrillation. In Circulation, 141, 301-312. doi:10.1161/CIRCULATIONAHA.119.044268. https://pubmed.ncbi.nlm.nih.gov/31735076/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen