C57BL/6JCya-Shc1em1/Cya
Common Name:
Shc1-KO
Product ID:
S-KO-18937
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Shc1-KO
Strain ID
KOCMP-20416-Shc1-B6J-VA
Gene Name
Product ID
S-KO-18937
Gene Alias
Shc; ShcA; p66; p66shc
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Shc1em1/Cya mice (Catalog S-KO-18937) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000094378
NCBI RefSeq
NM_001113331
Target Region
Exon 2~7
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Shc1, also known as Src homologous and collagen adaptor protein 1, is an adaptor protein that plays a pivotal role in multiple signal transduction pathways. It is involved in pathways such as the KRAS-MAPK signal transduction pathway and interacts with proteins like EGFR, AMPK, p38 MAPK, GRB2, and RET to regulate important cellular processes [1,2,3,4]. Shc1 is of great biological importance as it impacts cell proliferation, invasion, apoptosis, and mitosis, and is associated with various diseases including cancer, colitis, and non-obstructive azoospermia [1,2,6]. Genetic models, such as gene knockout models, can be valuable for studying its functions.
In lung cancer, Shc1 overexpression promotes cell proliferation and invasion, while knockout decreases these processes, indicating its carcinogenic role. Shc1 may interact with EGFR to form a protein complex, which could be a new target for lung cancer metastasis [1]. In colorectal cancer and colitis, miR-452 can regulate Shc1 expression, suggesting a potential defense mechanism during carcinogenesis or chronic inflammation [2]. In cancer cells, 8-Cl-cAMP treatment suppresses Shc1 phosphorylation, and Shc1 siRNA (a form of loss-of-function) induces AMPK and p38 MAPK phosphorylation and growth inhibition [3]. In clear cell renal cell carcinoma, USP15 stabilizes Shc1 via deubiquitination, promoting malignant biological properties [5]. Also, in non-obstructive azoospermia, SHC1 is identified as an immune-related oxidative stress biomarker [6].
In conclusion, Shc1 is an adaptor protein with key roles in multiple signal transduction pathways. Model-based research, especially through gene knockout-like experiments, has revealed its significance in various disease conditions such as cancer, colitis, and non-obstructive azoospermia. Understanding Shc1's functions provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Yang, Pan, Li, Wei, Li, Xiaoping. 2022. SHC1 Promotes Lung Cancer Metastasis by Interacting with EGFR. In Journal of oncology, 2022, 3599832. doi:10.1155/2022/3599832. https://pubmed.ncbi.nlm.nih.gov/35706930/
2. Mo, Ji-Su, Lamichhane, Santosh, Yun, Ki-Jung, Chae, Soo-Cheon. 2023. MicroRNA 452 regulates SHC1 expression in human colorectal cancer and colitis. In Genes & genomics, 45, 1295-1304. doi:10.1007/s13258-023-01432-3. https://pubmed.ncbi.nlm.nih.gov/37523129/
3. Choi, Ki Young, Cho, Young Jun, Kim, Jeong Seon, Ahn, Young-Ho, Hong, Seung Hwan. 2015. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment. In Biochemical and biophysical research communications, 463, 673-8. doi:10.1016/j.bbrc.2015.05.123. https://pubmed.ncbi.nlm.nih.gov/26043699/
4. Qiu, Ting, Kong, Yichao, Wei, Guifeng, Xie, Tian, Chen, Xiabin. 2024. CCDC6-RET fusion protein regulates Ras/MAPK signaling through the fusion- GRB2-SHC1 signal niche. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2322359121. doi:10.1073/pnas.2322359121. https://pubmed.ncbi.nlm.nih.gov/38805286/
5. Shi, Yaxing, Zhang, Jing, Li, Jiaxing, Yang, Da, Ju, Lincheng. 2024. USP15, activated by TFAP4 transcriptionally, stabilizes SHC1 via deubiquitination and deteriorates renal cell carcinoma. In Cancer science, 115, 2617-2629. doi:10.1111/cas.16237. https://pubmed.ncbi.nlm.nih.gov/38847328/
6. Pan, Yang, Chen, Xiangyu, Zhou, Hang, Liu, Li, Liu, Xiaoqiang. 2024. Identification and validation of SHC1 and FGFR1 as novel immune-related oxidative stress biomarkers of non-obstructive azoospermia. In Frontiers in endocrinology, 15, 1356959. doi:10.3389/fendo.2024.1356959. https://pubmed.ncbi.nlm.nih.gov/39391879/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen