C57BL/6JCya-Irf2em1/Cya
Common Name:
Irf2-KO
Product ID:
S-KO-18938
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Irf2-KO
Strain ID
KOCMP-16363-Irf2-B6J-VB
Gene Name
Product ID
S-KO-18938
Gene Alias
9830146E22Rik; Irf-2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Irf2em1/Cya mice (Catalog S-KO-18938) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034041
NCBI RefSeq
NM_008391
Target Region
Exon 3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Irf2, or interferon regulatory factor 2, is a transcription factor belonging to the IRF family. It plays diverse roles in multiple biological processes. It is involved in cytokine signaling, cell growth regulation, and hematopoietic development [4]. In the immune system, Irf2 is associated with interferon-mediated signaling pathways, which are crucial for immune activation and suppression [2,6].
In colorectal cancer, oncogenic KRASG12D (KRAS∗) represses Irf2 expression. This repression leads to high CXCL3 expression, promoting myeloid-derived suppressor cell migration to the tumor microenvironment and resulting in anti-PD-1 resistance. Enforced Irf2 expression can overcome this resistance [1]. In CD8+ T cells, deletion of Irf2 prevents T cell exhaustion within the tumor, enabling sustained effector functions and enhancing responsiveness to immune checkpoint and adoptive cell therapies [2]. In osteosarcoma, Irf2 negatively correlates with karyopherin alpha 2 (KPNA2). Irf2 binds to the KPNA2 promoter to modulate the malignant phenotypes of osteosarcoma cells [3]. For NK cells, knockdown of Irf2 greatly reduces the cell numbers of all early differentiation stages and impairs functional maturation [5]. In the context of myocardial infarction, IRF2 upregulation mediates gasdermin-D (GSDMD) induced pyroptosis [7]. Also, IRF2 is essential for GSDMD transcriptional activation, and its deficiency attenuates GSDMD expression, IL-1β secretion, and pyroptosis [8]. In melanomas, loss of Irf2 reduces MHC I pathway transcripts and causes resistance to checkpoint immunotherapy, but this can be reversed by interferon-induced IRF1 expression [9].
In conclusion, Irf2 has a wide-ranging impact on biological processes, especially in the immune system and in various diseases. Gene knockout or knockdown models in different cell types and animal models, such as mouse models, have been crucial in revealing its role in immune evasion, tumor development, cell differentiation, and pyroptosis-related pathologies. These findings provide potential targets for enhancing cancer control and treating other diseases like myocardial infarction.
References:
1. Liao, Wenting, Overman, Michael J, Boutin, Adam T, Wang, Y Alan, DePinho, Ronald A. 2019. KRAS-IRF2 Axis Drives Immune Suppression and Immune Therapy Resistance in Colorectal Cancer. In Cancer cell, 35, 559-572.e7. doi:10.1016/j.ccell.2019.02.008. https://pubmed.ncbi.nlm.nih.gov/30905761/
2. Lukhele, Sabelo, Rabbo, Diala Abd, Guo, Mengdi, McGaha, Tracy L, Brooks, David G. 2022. The transcription factor IRF2 drives interferon-mediated CD8+ T cell exhaustion to restrict anti-tumor immunity. In Immunity, 55, 2369-2385.e10. doi:10.1016/j.immuni.2022.10.020. https://pubmed.ncbi.nlm.nih.gov/36370712/
3. Xia, Shuchi, Ma, Yiqun. 2022. IRF2 Destabilizes Oncogenic KPNA2 to Modulate the Development of Osteosarcoma. In Journal of oncology, 2022, 9973519. doi:10.1155/2022/9973519. https://pubmed.ncbi.nlm.nih.gov/36199790/
4. Kim, Inyoung, Kim, Jung Ha, Kim, Kabsun, Lee, Keun-Bae, Kim, Nacksung. . IRF2 enhances RANKL-induced osteoclast differentiation via regulating NF-κB/NFATc1 signaling. In BMB reports, 54, 482-487. doi:. https://pubmed.ncbi.nlm.nih.gov/34488926/
5. Persyn, Eva, Wahlen, Sigrid, Kiekens, Laura, Van Vlierberghe, Pieter, Leclercq, Georges. 2022. IRF2 is required for development and functional maturation of human NK cells. In Frontiers in immunology, 13, 1038821. doi:10.3389/fimmu.2022.1038821. https://pubmed.ncbi.nlm.nih.gov/36544762/
6. Sheikh, Amania A, Utzschneider, Daniel T. . IRF2 integrates inflammatory signals to balance T cell exhaustion. In Immunity, 55, 2225-2227. doi:10.1016/j.immuni.2022.11.010. https://pubmed.ncbi.nlm.nih.gov/36516816/
7. Li, Yongxing, Wang, Yan, Guo, Hua, Wu, Qinghua, Hu, Yamin. 2021. IRF2 contributes to myocardial infarction via regulation of GSDMD induced pyroptosis. In Molecular medicine reports, 25, . doi:10.3892/mmr.2021.12556. https://pubmed.ncbi.nlm.nih.gov/34878155/
8. Kayagaki, Nobuhiko, Lee, Bettina L, Stowe, Irma B, Bertram, Edward M, Dixit, Vishva M. 2019. IRF2 transcriptionally induces GSDMD expression for pyroptosis. In Science signaling, 12, . doi:10.1126/scisignal.aax4917. https://pubmed.ncbi.nlm.nih.gov/31113851/
9. Sari, G, Dhatchinamoorthy, K, Orellano-Ariza, L, Brehm, M A, Rock, K. 2024. IRF2 loss is associated with reduced MHC I pathway transcripts in subsets of most human cancers and causes resistance to checkpoint immunotherapy in human and mouse melanomas. In Journal of experimental & clinical cancer research : CR, 43, 276. doi:10.1186/s13046-024-03187-5. https://pubmed.ncbi.nlm.nih.gov/39354629/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen