C57BL/6JCya-Sorbs2em1/Cya
Common Name:
Sorbs2-KO
Product ID:
S-KO-18947
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Sorbs2-KO
Strain ID
KOCMP-234214-Sorbs2-B6J-VA
Gene Name
Product ID
S-KO-18947
Gene Alias
2010203O03Rik; 9430041O17Rik; A530071H08; Argbp2; mKIAA0777; nArgBP2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sorbs2em1/Cya mice (Catalog S-KO-18947) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000171337
NCBI RefSeq
NM_001205219
Target Region
Exon 8
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Sorbs2, also known as Sorbin and SH3 Domain Containing 2, is ubiquitously expressed in various tissues including the cardiovascular system. It serves as a cytoskeletal adaptor and an RNA-binding protein. Sorbs2 is involved in regulating the expression and function of proteins like the BK channel in vascular smooth muscle cells, and may be associated with pathways related to inflammation, lipid metabolism, and synaptic transmission [1]. Its function is crucial for maintaining normal physiological processes in the body, and genetic models such as KO mouse models are valuable for studying its role.
In KO mouse models, Sorbs2 knockout in cardiomyocytes leads to dilated cardiomyopathy, with defective microtubule polymerization and compensatory upregulation of structural cytoskeletal and adapter proteins responsible for contractile dysfunction [3]. Sorbs2 knockout mice at 4 months also display a decrease in BK channel expression and function in coronary arteries, mimicking the vasculopathy seen in diabetic mice [1]. In human embryonic stem cell differentiation models, knockdown of SORBS2 disrupts sarcomeric integrity of cardiomyocytes and reduces cardiomyocyte number [2].
In conclusion, Sorbs2 is essential for maintaining the structural integrity of cardiomyocytes and regulating BK channel function in vascular smooth muscle cells. Studies using KO mouse models have revealed its significant role in cardiovascular diseases, including dilated cardiomyopathy and diabetic vasculopathy, providing insights into the mechanisms of these diseases and potential therapeutic targets.
References:
1. Sun, Xiaojing, Lee, Hon-Chi, Lu, Tong. 2024. Sorbs2 Deficiency and Vascular BK Channelopathy in Diabetes. In Circulation research, 134, 858-871. doi:10.1161/CIRCRESAHA.123.323538. https://pubmed.ncbi.nlm.nih.gov/38362769/
2. Liang, Fei, Wang, Bo, Geng, Juan, Zhang, Xiaoqing, Zhang, Zhen. 2021. SORBS2 is a genetic factor contributing to cardiac malformation of 4q deletion syndrome patients. In eLife, 10, . doi:10.7554/eLife.67481. https://pubmed.ncbi.nlm.nih.gov/34099102/
3. McLendon, Jared M, Zhang, Xiaoming, Matasic, Daniel S, London, Barry, Boudreau, Ryan L. 2022. Knockout of Sorbin And SH3 Domain Containing 2 (Sorbs2) in Cardiomyocytes Leads to Dilated Cardiomyopathy in Mice. In Journal of the American Heart Association, 11, e025687. doi:10.1161/JAHA.122.025687. https://pubmed.ncbi.nlm.nih.gov/35730644/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen