C57BL/6JCya-Tmc1em1/Cya
Common Name:
Tmc1-KO
Product ID:
S-KO-18952
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Tmc1-KO
Strain ID
KOCMP-13409-Tmc1-B6J-VB
Gene Name
Product ID
S-KO-18952
Gene Alias
4933416G09Rik; Beethoven; Bth; CWEA1; TMC1ex1; dn
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tmc1em1/Cya mice (Catalog S-KO-18952) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000039500
NCBI RefSeq
NM_028953
Target Region
Exon 4
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Tmc1, short for Transmembrane channel-like protein isoform-1, is a prime candidate for the mechano-electrical transducer (MET) channel in hair cells of the inner ear. It is thought to have a six-transmembrane domain structure and localizes to the tips of stereocilia in the sensory hair bundle, where the MET channel is located. Mutations in Tmc1 are linked to human deafness, highlighting its crucial role in auditory function [1].
Tmc1 mutant mice have been used to study its function. For example, in Tmc1;Tmc2 double knockout mice, mechanotransduction currents are lost in hair cells, indicating the involvement of Tmc1 in auditory mechanotransduction [3]. Also, specific Tmc1 mutations in mice, like Tmc1 p.T416K and others, cause deafness and outer hair cell loss, and affect MET channel properties such as Ca2+ permeability, resting open probability, and single-channel conductance [2].
In conclusion, Tmc1 is essential for the mechanotransduction process in cochlear hair cells, which is fundamental for hearing. Studies using Tmc1 mutant mouse models have significantly advanced our understanding of how Tmc1 mutations lead to deafness, providing insights into the molecular mechanisms underlying auditory function and potential therapeutic targets for hearing loss [2,3].
References:
1. Fettiplace, Robert. . Is TMC1 the Hair Cell Mechanotransducer Channel? In Biophysical journal, 111, 3-9. doi:10.1016/j.bpj.2016.05.032. https://pubmed.ncbi.nlm.nih.gov/27410728/
2. Beurg, Maryline, Schimmenti, Lisa A, Koleilat, Alaa, Ballesteros, Angela, Fettiplace, Robert. 2021. New Tmc1 Deafness Mutations Impact Mechanotransduction in Auditory Hair Cells. In The Journal of neuroscience : the official journal of the Society for Neuroscience, 41, 4378-4391. doi:10.1523/JNEUROSCI.2537-20.2021. https://pubmed.ncbi.nlm.nih.gov/33824189/
3. Deng, Siqi, Yan, Zhiqiang. 2025. TMC1 and TMC2 function as the mechano-electrical transduction ion channel in hearing. In Current opinion in neurobiology, 93, 103026. doi:10.1016/j.conb.2025.103026. https://pubmed.ncbi.nlm.nih.gov/40280017/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen