C57BL/6JCya-Col15a1em1/Cya
Common Name:
Col15a1-KO
Product ID:
S-KO-18971
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Col15a1-KO
Strain ID
KOCMP-12819-Col15a1-B6J-VB
Gene Name
Product ID
S-KO-18971
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Col15a1em1/Cya mice (Catalog S-KO-18971) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102917
NCBI RefSeq
NM_009928
Target Region
Exon 7
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Col15a1 encodes the alpha chain of collagen XV, a member of the FACIT collagen family [1]. It has anti-angiogenic and anti-tumoral properties and is involved in tissue homeostasis in the liver [1]. In the context of vascular-related functions, it may be associated with processes like vasculogenesis [2].
In the mouse mammary carcinoma model, inactivation of Col15a1 leads to changes in the fibrillar tumour matrix and increased mammary tumour growth, supporting its tumour suppressor function in mammary carcinogenesis [3]. In smooth muscle cell (SMC) proliferation, hypomethylation of Col15a1 occurs, and the consequent increased gene expression may impact SMC phenotype and atherosclerosis formation [4]. Also, in canine endothelial-like cells, miR-9 promotes cell migration by inhibiting the expression of collagen XV encoded by Col15a1 [5].
In conclusion, Col15a1 plays essential roles in various biological processes, especially in tumour-related and vascular-related functions. Studies using mouse models, like the inactivation of Col15a1 in the mammary carcinoma model, have revealed its tumour-suppressing effect in breast carcinogenesis. Its role in SMC-related processes also indicates its significance in atherosclerosis-related diseases. These findings enhance our understanding of the biological functions of Col15a1 and its implications in disease mechanisms.
References:
1. Wang, Shuomin, Zhou, Mingran, Xia, Yunhong. 2023. COL15A1 interacts with P4HB to regulate the growth and malignancy of HepG2.2.15 cells. In Biochemical and biophysical research communications, 681, 20-28. doi:10.1016/j.bbrc.2023.09.031. https://pubmed.ncbi.nlm.nih.gov/37742474/
2. Sudduth, Christopher L, Smits, Patrick J, Vivero, Matthew P, Warman, Matthew L, Greene, Arin K. 2023. Arteriovenous malformation Map2k1 mutation affects vasculogenesis. In Scientific reports, 13, 11074. doi:10.1038/s41598-023-35301-6. https://pubmed.ncbi.nlm.nih.gov/37422456/
3. Martínez-Nieto, Guillermo, Heljasvaara, Ritva, Heikkinen, Anne, Pihlajaniemi, Taina, Karppinen, Sanna-Maria. 2021. Deletion of Col15a1 Modulates the Tumour Extracellular Matrix and Leads to Increased Tumour Growth in the MMTV-PyMT Mouse Mammary Carcinoma Model. In International journal of molecular sciences, 22, . doi:10.3390/ijms22189978. https://pubmed.ncbi.nlm.nih.gov/34576139/
4. Connelly, Jessica J, Cherepanova, Olga A, Doss, Jennifer F, Hauser, Elizabeth R, Gregory, Simon G. 2013. Epigenetic regulation of COL15A1 in smooth muscle cell replicative aging and atherosclerosis. In Human molecular genetics, 22, 5107-20. doi:10.1093/hmg/ddt365. https://pubmed.ncbi.nlm.nih.gov/23912340/
5. Jiang, Heng, Liu, Mengmeng, Qin, Yao, Zhang, Hong. 2023. miR-9 promotes canine endothelial-like cell migration by targeting COL15A1. In Veterinary medicine and science, 10, e1339. doi:10.1002/vms3.1339. https://pubmed.ncbi.nlm.nih.gov/38109263/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen