C57BL/6JCya-Srrtem1/Cya
Common Name:
Srrt-KO
Product ID:
S-KO-19057
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Srrt-KO
Strain ID
KOCMP-83701-Srrt-B6J-VA
Gene Name
Product ID
S-KO-19057
Gene Alias
2810019G02Rik; ASR2A; ASR2B; ASR2C; ASR2D; Ars2; Asr2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Srrtem1/Cya mice (Catalog S-KO-19057) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000199243
NCBI RefSeq
NM_001109909
Target Region
Exon 3~9
Size of Effective Region
~3.5 kb
Detailed Document
Overview of Gene Research
Srrt, also known as ARS2 (arsenite-resistance protein 2), is an essential eukaryotic protein. It is at the nexus of RNA polymerase II (Pol II) transcription, transcript maturation and quality control. Through interacting with the Cap Binding Complex (CBC), it associates with the cap of new RNAs, acting as a hub for protein factor exchanges that determine RNA fate. It is involved in maintaining the identity of various cell types, such as embryonic stem cells and neural stem cells, and may play a role in regulating the mitochondrial proteome [1,3,4,5].
In neural stem cells, selective knockdown of Ars2 (Srrt) in glial fibrillary acidic protein-expressing cells in the adult subventricular zone of mice depleted the number of neural stem cells and their neurogenic capacity. These phenotypes were recapitulated in hGFAP-cre::Ars2(fl/fl) conditional knockout mice, with more severe effects. Ex vivo assays showed that Ars2 was necessary and sufficient to promote neural stem cell self-renewal by positively regulating the expression of Sox2 [5]. In embryonic stem cells, Srrt sustains cell identity by preventing premature termination of numerous transcripts at cryptic cleavage/polyadenylation sites in first introns [4].
In conclusion, Srrt is crucial for maintaining cell identity in neural and embryonic stem cells through its roles in transcription and transcript processing. The gene knockout and conditional knockout mouse models have revealed its function in self-renewal and transcript regulation, which may have implications for understanding diseases related to stem cell function, such as certain neurological disorders and potentially cancer, given its association with prostate cancer progression [2,5].
References:
1. Lykke-Andersen, Søren, Rouvière, Jérôme O, Jensen, Torben Heick. . ARS2/SRRT: at the nexus of RNA polymerase II transcription, transcript maturation and quality control. In Biochemical Society transactions, 49, 1325-1336. doi:10.1042/BST20201008. https://pubmed.ncbi.nlm.nih.gov/34060620/
2. Gamallat, Yaser, Choudhry, Muhammad, Li, Qiaowang, Boutros, Paul C, Bismar, Tarek A. 2023. Serrate RNA Effector Molecule (SRRT) Is Associated with Prostate Cancer Progression and Is a Predictor of Poor Prognosis in Lethal Prostate Cancer. In Cancers, 15, . doi:10.3390/cancers15102867. https://pubmed.ncbi.nlm.nih.gov/37345203/
3. Meisel, Joshua D, Wiesenthal, Presli P, Mootha, Vamsi K, Ruvkun, Gary. 2024. CMTR-1 RNA methyltransferase mutations activate widespread expression of a dopaminergic neuron-specific mitochondrial complex I gene. In Current biology : CB, 34, 2728-2738.e6. doi:10.1016/j.cub.2024.04.079. https://pubmed.ncbi.nlm.nih.gov/38810637/
4. Kainov, Yaroslav A, Makeyev, Eugene V. 2020. A transcriptome-wide antitermination mechanism sustaining identity of embryonic stem cells. In Nature communications, 11, 361. doi:10.1038/s41467-019-14204-z. https://pubmed.ncbi.nlm.nih.gov/31953406/
5. Andreu-Agullo, Celia, Maurin, Thomas, Thompson, Craig B, Lai, Eric C. 2011. Ars2 maintains neural stem-cell identity through direct transcriptional activation of Sox2. In Nature, 481, 195-8. doi:10.1038/nature10712. https://pubmed.ncbi.nlm.nih.gov/22198669/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen