C57BL/6JCya-Npr1em1/Cya
Common Name:
Npr1-KO
Product ID:
S-KO-19064
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Npr1-KO
Strain ID
KOCMP-18160-Npr1-B6J-VA
Gene Name
Product ID
S-KO-19064
Gene Alias
GC-A; NPR-A; NPRA; Pndr
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Npr1em1/Cya mice (Catalog S-KO-19064) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029540
NCBI RefSeq
NM_008727
Target Region
Exon 2~7
Size of Effective Region
~4.0 kb
Detailed Document
Overview of Gene Research
Npr1, also known as Nonexpressor of pathogenesis-related genes 1, is a master regulator in plants. It is central to the salicylic acid (SA)-mediated systemic acquired resistance (SAR), a broad-spectrum disease resistance mechanism. NPR1 controls about 90% of SA-dependent transcriptome in Arabidopsis and plays a crucial role in plant survival under biotic and abiotic stresses [2,6].
NPR1 promotes cell survival during the plant immune response by forming salicylic acid-induced NPR1 condensates (SINCs). These SINCs are enriched with stress-response proteins. The transition of NPR1 into condensates is required for the formation of the NPR1-Cullin 3 E3 ligase complex, which ubiquitinates SINC-localized substrates like EDS1 and specific WRKY transcription factors [1]. Also, NPR1 is a SA receptor. SA-bound NPR1 dimer activates transcription by bridging two TGA transcription factor dimers to form an enhanceosome. Moreover, NPR1 functions in plant immunity through forming distinct nuclear and cytoplasmic biomolecular condensates [2,3]. In addition, both NPR1 and NPR3/NPR4 are SA receptors, but they play opposite roles in transcriptional regulation of SA-induced defense gene expression [4]. The perception of SA by NPR1 and NPR4 is needed for the activation of N-hydroxypipecolic acid biosynthesis, which is essential for inducing systemic acquired resistance [5]. High air humidity dampens the SA pathway and NPR1 function, as NPR1 is less ubiquitinated and has lower promoter binding affinity under high humidity [7]. A fungal effector CfEC12 can target the plant SA defense pathway by suppressing the interaction between MdNIMIN2 and MdNPR1, thus promoting fungal infection [8]. Overexpressing NPR1 in transgenic plants can enhance resistance to biotic and abiotic stresses, but may also cause detrimental morphological traits [9].
In summary, NPR1 is a key regulator in plant immunity, mainly through its functions in SA-mediated signaling, transcriptional regulation, and formation of condensates. Understanding NPR1's role helps in developing strategies to engineer plant resistance against biotic and abiotic stresses.
References：
1. Zavaliev, Raul, Mohan, Rajinikanth, Chen, Tianyuan, Dong, Xinnian. 2020. Formation of NPR1 Condensates Promotes Cell Survival during the Plant Immune Response. In Cell, 182, 1093-1108.e18. doi:10.1016/j.cell.2020.07.016. https://pubmed.ncbi.nlm.nih.gov/32810437/
2. Zavaliev, Raul, Dong, Xinnian. 2023. NPR1, a key immune regulator for plant survival under biotic and abiotic stresses. In Molecular cell, 84, 131-141. doi:10.1016/j.molcel.2023.11.018. https://pubmed.ncbi.nlm.nih.gov/38103555/
3. Kumar, Shivesh, Zavaliev, Raul, Wu, Qinglin, Dong, Xinnian, Zhou, Pei. 2022. Structural basis of NPR1 in activating plant immunity. In Nature, 605, 561-566. doi:10.1038/s41586-022-04699-w. https://pubmed.ncbi.nlm.nih.gov/35545668/
4. Ding, Yuli, Sun, Tongjun, Ao, Kevin, Li, Xin, Zhang, Yuelin. 2018. Opposite Roles of Salicylic Acid Receptors NPR1 and NPR3/NPR4 in Transcriptional Regulation of Plant Immunity. In Cell, 173, 1454-1467.e15. doi:10.1016/j.cell.2018.03.044. https://pubmed.ncbi.nlm.nih.gov/29656896/
5. Liu, Yanan, Sun, Tongjun, Sun, Yulin, Li, Xin, Zhang, Yuelin. 2020. Diverse Roles of the Salicylic Acid Receptors NPR1 and NPR3/NPR4 in Plant Immunity. In The Plant cell, 32, 4002-4016. doi:10.1105/tpc.20.00499. https://pubmed.ncbi.nlm.nih.gov/33037144/
6. Sun, Yali, Detchemendy, Thomas Ward, Pajerowska-Mukhtar, Karolina Marta, Mukhtar, M Shahid. 2018. NPR1 in JazzSet with Pathogen Effectors. In Trends in plant science, 23, 469-472. doi:10.1016/j.tplants.2018.04.007. https://pubmed.ncbi.nlm.nih.gov/29753632/
7. Yao, Lingya, Jiang, Zeyu, Wang, Yiping, Wan, Shiwei, Xin, Xiu-Fang. 2023. High air humidity dampens salicylic acid pathway and NPR1 function to promote plant disease. In The EMBO journal, 42, e113499. doi:10.15252/embj.2023113499. https://pubmed.ncbi.nlm.nih.gov/37728254/
8. Shang, Shengping, Liu, Guangli, Zhang, Song, Zhang, Rong, Sun, Guangyu. 2023. A fungal CFEM-containing effector targets NPR1 regulator NIMIN2 to suppress plant immunity. In Plant biotechnology journal, 22, 82-97. doi:10.1111/pbi.14166. https://pubmed.ncbi.nlm.nih.gov/37596985/
9. Silva, Katchen Julliany P, Mahna, Nasser, Mou, Zhonglin, Folta, Kevin M. 2018. NPR1 as a transgenic crop protection strategy in horticultural species. In Horticulture research, 5, 15. doi:10.1038/s41438-018-0026-1. https://pubmed.ncbi.nlm.nih.gov/29581883/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen