C57BL/6JCya-Fbxo9em1/Cya
Common Name:
Fbxo9-KO
Product ID:
S-KO-19066
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Fbxo9-KO
Strain ID
KOCMP-71538-Fbxo9-B6J-VB
Gene Name
Product ID
S-KO-19066
Gene Alias
9030401P18Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fbxo9em1/Cya mice (Catalog S-KO-19066) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001402
NCBI RefSeq
NM_001081490
Target Region
Exon 4~5
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Fbxo9, an F-box protein, serves as a substrate receptor for the SKP1-cullin-1-RBX1 ubiquitin ligase. It is involved in multiple cellular processes such as cell proliferation, apoptosis, migration, and is associated with pathways like V-ATPase assembly, Wnt signaling, and the ubiquitin-proteasome system [1,2,4]. It has significance in various biological contexts including cancer, cell fate determination, and adipocyte differentiation, making genetic models crucial for its study.
In cancer, loss-of-function experiments have shown diverse effects. In lung cancer, knockdown of Fbxo9 increased V-ATPase assembly, vesicular acidification, pro-metastatic Wnt signaling, and metastasis, indicating its tumor-suppressive role [1]. In hepatocellular carcinoma, FBXO9 facilitated cell proliferation and metastasis, and its knockdown increased sensitivity to drugs [2]. In acute myeloid leukemia, deletion of Fbxo9 in a murine hematopoietic system using a Nuclease technology-based conditional knockout mouse model led to accelerated and aggressive leukemia development, and tumors lacking Fbxo9 had increased proteasome activity [3]. In osteoclasts, knockdown of FBXO9 induced ferroptosis [5].
In conclusion, Fbxo9 plays essential roles in regulating multiple biological processes. Through gene knockout and conditional knockout mouse models, its role in diseases like cancer and cell-specific functions has been revealed. These studies on Fbxo9 enhance our understanding of biological mechanisms and may provide potential therapeutic targets for related diseases.
References:
1. Liu, Liang, Chen, Xiaodong, Wu, Leilei, Wei, Dongping, Xu, Yaping. 2024. Ubiquitin ligase subunit FBXO9 inhibits V-ATPase assembly and impedes lung cancer metastasis. In Experimental hematology & oncology, 13, 32. doi:10.1186/s40164-024-00497-4. https://pubmed.ncbi.nlm.nih.gov/38486234/
2. Wang, Zhenyu, Chen, Xiaoxia, Zhou, Lianer, Li, Hong, Li, Jinjun. 2022. FBXO9 Mediates the Cancer-Promoting Effects of ZNF143 by Degrading FBXW7 and Facilitates Drug Resistance in Hepatocellular Carcinoma. In Frontiers in oncology, 12, 930220. doi:10.3389/fonc.2022.930220. https://pubmed.ncbi.nlm.nih.gov/35847937/
3. Hynes-Smith, R Willow, Swenson, Samantha A, Vahle, Heather, Hyde, R Katherine, Buckley, Shannon M. 2019. Loss of FBXO9 Enhances Proteasome Activity and Promotes Aggressiveness in Acute Myeloid Leukemia. In Cancers, 11, . doi:10.3390/cancers11111717. https://pubmed.ncbi.nlm.nih.gov/31684170/
4. Hussain, Shujaat, Dong, Jianshu, Ma, Xinli, Clement, Agboybor, Liu, Hongmin. 2022. F-box only protein 9 and its role in cancer. In Molecular biology reports, 49, 1537-1544. doi:10.1007/s11033-021-07057-7. https://pubmed.ncbi.nlm.nih.gov/35025031/
5. Qu, Xingzhou, Sun, Zhaoqi, Wang, Yang, Ong, Hui Shan. 2021. Zoledronic acid promotes osteoclasts ferroptosis by inhibiting FBXO9-mediated p53 ubiquitination and degradation. In PeerJ, 9, e12510. doi:10.7717/peerj.12510. https://pubmed.ncbi.nlm.nih.gov/35003915/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen