C57BL/6JCya-Pon1em1/Cya
Common Name:
Pon1-KO
Product ID:
S-KO-19069
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Pon1-KO
Strain ID
KOCMP-18979-Pon1-B6J-VA
Gene Name
Product ID
S-KO-19069
Gene Alias
Pon
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pon1em1/Cya mice (Catalog S-KO-19069) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000002663
NCBI RefSeq
NM_011134
Target Region
Exon 2~4
Size of Effective Region
~3.4 kb
Detailed Document
Overview of Gene Research
PON1, short for paraoxonase 1, is a high-density lipoprotein-associated enzyme with antioxidant, anti-inflammatory, and anti-apoptotic roles [6]. One of its most important enzymatic activities in association with high-density lipoprotein (HDL) is the prevention of low-density lipoprotein (LDL) oxidation, giving it an anti-atherogenic activity [2]. It is also involved in the detoxification of organophosphate insecticides [3,7].
PON1 gene polymorphisms have been widely studied. The PON1 L55M polymorphism might increase the risk of cancer, especially breast cancer in certain populations, and the PON1 Q192R and L55M polymorphisms may increase the risk of organophosphate toxicity, particularly among Caucasian populations [1,3]. In addition, the PON1 Q192R polymorphism has a weak association with coronary heart disease risk in Chinese [8]. Liver disease significantly reduces PON1 activity, and the gene polymorphism is associated with an increased risk of liver disease [4]. In idiopathic inflammatory myopathies, the arylesterase and lactonase activities of PON1 are significantly impaired and inversely associate with disease activity [5].
In conclusion, PON1 is crucial for maintaining normal physiological functions, especially in lipid metabolism, detoxification, and protection against oxidative stress. Its gene polymorphisms are associated with various diseases such as cancer, organophosphate toxicity-related conditions, coronary heart disease, liver disease, and idiopathic inflammatory myopathies. Research on PON1 helps in understanding disease mechanisms and may offer potential for targeted therapies.
References:
1. Hu, Peizeng, Ma, Yongchen, Zhang, Lingling, Ma, Sheng. 2016. PON1 L55M polymorphism might contribute to the risk of cancer. In Panminerva medica, 59, 107-113. doi:10.23736/S0031-0808.16.03212-2. https://pubmed.ncbi.nlm.nih.gov/27309259/
2. Tajbakhsh, Amir, Rezaee, Mehdi, Rivandi, Mahdi, Afzaljavan, Fahimeh, Pasdar, Alireza. 2017. Paraoxonase 1 (PON1) and stroke; the dilemma of genetic variation. In Clinical biochemistry, 50, 1298-1305. doi:10.1016/j.clinbiochem.2017.08.001. https://pubmed.ncbi.nlm.nih.gov/28779954/
3. You, Tian, Lv, Jingyu, Zhou, Liping. 2013. PON1 Q192R and L55M polymorphisms and organophosphate toxicity risk: a meta-analysis. In DNA and cell biology, 32, 252-9. doi:10.1089/dna.2012.1961. https://pubmed.ncbi.nlm.nih.gov/23590198/
4. Salari, Nader, Kazeminia, Mohsen, Mansouri, Kamran, Hosseinian-Far, Amin, Mohammadi, Masoud. 2021. The Activity and Polymorphism of the PON1 in Patients with Chronic Liver Disease: a Systematic Review and Meta-analysis. In Journal of gastrointestinal cancer, 53, 745-755. doi:10.1007/s12029-021-00699-7. https://pubmed.ncbi.nlm.nih.gov/34467517/
5. Bae, Sangmee Sharon, Shahbazian, Ani, Wang, Jennifer, Reddy, Srinivasa T, Charles-Schoeman, Christina. . Abnormal paraoxonase-1 (PON1) enzyme activity in idiopathic inflammatory myopathies. In Rheumatology (Oxford, England), 61, 2512-2523. doi:10.1093/rheumatology/keab795. https://pubmed.ncbi.nlm.nih.gov/34698804/
6. Marsillach, Judit, Richter, Rebecca J, Costa, Lucio G, Furlong, Clement E. . Paraoxonase-1 (PON1) Status Analysis Using Non-Organophosphate Substrates. In Current protocols, 1, e25. doi:10.1002/cpz1.25. https://pubmed.ncbi.nlm.nih.gov/33484495/
7. Costa, Lucio G, Giordano, Gennaro, Cole, Toby B, Marsillach, Judit, Furlong, Clement E. 2012. Paraoxonase 1 (PON1) as a genetic determinant of susceptibility to organophosphate toxicity. In Toxicology, 307, 115-22. doi:10.1016/j.tox.2012.07.011. https://pubmed.ncbi.nlm.nih.gov/22884923/
8. Zhang, Zhen, Ou, Junke, Cai, Peiling, Niu, Bei, Li, Jian. . Association between the PON1 Q192R polymorphism and coronary heart disease in Chinese: A meta-analysis. In Medicine, 97, e11151. doi:10.1097/MD.0000000000011151. https://pubmed.ncbi.nlm.nih.gov/29952962/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen