C57BL/6JCya-Prelid1em1/Cya
Common Name:
Prelid1-KO
Product ID:
S-KO-19095
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Prelid1-KO
Strain ID
KOCMP-66494-Prelid1-B6J-VA
Gene Name
Product ID
S-KO-19095
Gene Alias
2610524G07Rik; Preli
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
13
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prelid1em1/Cya mice (Catalog S-KO-19095) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021942
NCBI RefSeq
NM_025596
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
PRELID1, the only late embryogenesis abundant (LEA) domain-containing protein in humans, is a lipid transfer protein in the Ups/PRELI family. It facilitates the transport of phosphatidic acid (PA) across the mitochondrial intermembrane space, which is crucial for the conversion of PA to cardiolipin (CL), a signature phospholipid of mitochondria. This process is essential for maintaining mitochondrial structure and function. PRELID1 also plays roles in stress response and is associated with pathways like the unfolded protein response (UPR) and mTORC1 signaling [3,4,5].
In breast cancer, an alternative polyadenylation (APA) event in PRELID1 mRNA enhances its steady-state level and translational efficiency, and is a strong breast-cancer-subtype-dependent predictor of patient clinical outcomes. PRELID1 also regulates stress response and mitochondrial reactive oxygen species (ROS) production in a cell-type-specific manner. In mesenchymal stem cell-treated thymic epithelial cells, decreased methylation of PRELID1 promoter leads to its up-regulation, promoting cell growth [1,2]. In yeast lacking Ups1 (a homolog of PRELID1), disturbed intramitochondrial PA transport impairs glycolytic growth and cellular stress signaling, such as UPR and mTORC1 signaling [3].
In summary, PRELID1 is vital for mitochondrial lipid transport and stress-related cellular functions. Its study, especially through model-based research like in yeast models similar to Ups1-deficient yeast, has revealed its significance in cancer and cell growth-related disease areas. In cancer, it is associated with patient outcomes and mitochondrial ROS signaling, while in thymus aging, it is involved in the regulation of cell growth through DNA methylation-related mechanisms [1,2,3].
References:
1. Gillen, Austin E, Brechbuhl, Heather M, Yamamoto, Tomomi M, Hesselberth, Jay R, Kabos, Peter. 2017. Alternative Polyadenylation of PRELID1 Regulates Mitochondrial ROS Signaling and Cancer Outcomes. In Molecular cancer research : MCR, 15, 1741-1751. doi:10.1158/1541-7786.MCR-17-0010. https://pubmed.ncbi.nlm.nih.gov/28912168/
2. Yang, Zailing, Tian, Chuan, He, Zhixu, Wu, XiJun, Pan, Xinghua. 2024. Mesenchymal stem cells reverse thymus aging by reprogramming the DNA methylation of thymic epithelial cells. In Regenerative therapy, 27, 126-169. doi:10.1016/j.reth.2024.03.008. https://pubmed.ncbi.nlm.nih.gov/38571892/
3. Eiyama, Akinori, Aaltonen, Mari J, Nolte, Hendrik, Tatsuta, Takashi, Langer, Thomas. 2021. Disturbed intramitochondrial phosphatidic acid transport impairs cellular stress signaling. In The Journal of biological chemistry, 296, 100335. doi:10.1016/j.jbc.2021.100335. https://pubmed.ncbi.nlm.nih.gov/33497623/
4. Hall, Brandon M, Owens, Kjerstin M, Singh, Keshav K. 2011. Distinct functions of evolutionary conserved MSF1 and late embryogenesis abundant (LEA)-like domains in mitochondria. In The Journal of biological chemistry, 286, 39141-52. doi:10.1074/jbc.M111.259853. https://pubmed.ncbi.nlm.nih.gov/21930701/
5. Miliara, Xeni, Tatsuta, Takashi, Berry, Jamie-Lee, Matthews, Stephen, Langer, Thomas. 2019. Structural determinants of lipid specificity within Ups/PRELI lipid transfer proteins. In Nature communications, 10, 1130. doi:10.1038/s41467-019-09089-x. https://pubmed.ncbi.nlm.nih.gov/30850607/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen