C57BL/6JCya-Xpo5em1/Cya
Common Name
Xpo5-KO
Product ID
S-KO-19121
Backgroud
C57BL/6JCya
Strain ID
KOCMP-72322-Xpo5-B6J-VB
Status
When using this mouse strain in a publication, please cite “Xpo5-KO Mouse (Catalog S-KO-19121) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Xpo5-KO
Strain ID
KOCMP-72322-Xpo5-B6J-VB
Gene Name
Product ID
S-KO-19121
Gene Alias
Exp5, RanBp21, mKIAA1291, 2410004H11Rik, 2700038C24Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 17
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000087031
NCBI RefSeq
NM_028198
Target Region
Exon 6~8
Size of Effective Region
~3.2 kb
Overview of Gene Research
XPO5, also known as Exportin 5, is a key protein in the miRNA biogenesis pathway. It mediates the nuclear export of precursor pre-miRNA to the cytoplasm in a RanGTP-dependent manner, which is crucial for the maturation of miRNAs that play vital roles in gene expression regulation and cell homeostasis [1,2].
Genetic deletion of XPO5 in mouse models compromises the biogenesis of most miRNAs, leading to severe defects during mouse embryonic development and skin morphogenesis, revealing its essential role in these biological processes [2]. In addition, in vitro studies show that knockdown of XPO5 in head and neck cancer cells decreases cell proliferation, delays wound healing, and increases caspase-3 enzyme activity, indicating its oncogenic potential in head and neck squamous cell carcinoma (HNSCC) [3].
In conclusion, XPO5 is essential for miRNA biogenesis and plays important roles in various biological processes such as embryonic development and skin morphogenesis. Its dysregulation is associated with diseases like HNSCC. The study of XPO5 knockout or knockdown models has provided valuable insights into its functions and its implications in disease, offering potential directions for biomarker discovery and therapeutic target identification.
References：
1. Patrão, Ana Sofia, Dias, Francisca, Teixeira, Ana Luísa, Maurício, Joaquina, Medeiros, Rui. 2018. XPO5 genetic polymorphisms in cancer risk and prognosis. In Pharmacogenomics, 19, 799-808. doi:10.2217/pgs-2018-0018. https://pubmed.ncbi.nlm.nih.gov/29790454/
2. Wang, Jingjing, Lee, Jerome E, Riemondy, Kent, Lai, Eric C, Yi, Rui. 2020. XPO5 promotes primary miRNA processing independently of RanGTP. In Nature communications, 11, 1845. doi:10.1038/s41467-020-15598-x. https://pubmed.ncbi.nlm.nih.gov/32296071/
3. Özdaş, Sibel, Canatar, İpek, Özdaş, Talih. 2021. Effects of Knockdown of XPO5 by siRNA on the Biological Behavior of Head and Neck Cancer Cells. In The Laryngoscope, 132, 569-577. doi:10.1002/lary.29787. https://pubmed.ncbi.nlm.nih.gov/34328643/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.