C57BL/6JCya-Haus1em1/Cya
Common Name:
Haus1-KO
Product ID:
S-KO-19128
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Haus1-KO
Strain ID
KOCMP-225745-Haus1-B6J-VB
Gene Name
Product ID
S-KO-19128
Gene Alias
Ccdc5; HEI-C
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Haus1em1/Cya mice (Catalog S-KO-19128) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000236234
NCBI RefSeq
NM_001357532
Target Region
Exon 3~10
Size of Effective Region
~10.1 kb
Detailed Document
Overview of Gene Research
HAUS1, also known as HAUS Augmin-like complex subunit 1, is a key component of the augmin complex, which is crucial for branched microtubule nucleation during mitotic spindle assembly [4]. This process is directly regulated by the Ran-GTP pathway, where importins and Ran-GTP control the microtubule binding of the augmin complex [4].
In various cancers, HAUS1 shows differential expression patterns. In hepatocellular carcinoma (HCC), it is highly expressed, leading to a poor prognosis, and is associated with clinical stage, pathological grade, and AFP. It also has diagnostic value and is an independent prognostic factor. Additionally, it is linked to immune cell infiltration and immune checkpoints in HCC, and may generate significant therapeutic results when combined with anti-CTLA4 and anti-CD274 treatment [1].
In prostate adenocarcinoma (PRAD), HAUS1 is downregulated compared to normal tissues, and can serve as an independent indicator for overall survival prognosis. It is closely related to the tumor microenvironment, immune cell infiltration levels, immune checkpoints, and immune cell pathways, as well as the immunotherapeutic response of patients [2].
In glioma, an elevated expression of HAUS1 is observed, associated with poorer patient survival, unfavorable clinical characteristics, and is an independent predictor. It is also associated with several tumour-infiltrating immune cells [3].
In male infertility, spermatozoa from the poor early embryo development subgroup display mutations impacting centrosome integrity related to HAUS1 [5].
In conclusion, HAUS1 is essential for microtubule-related processes like spindle assembly. Its dysregulation is implicated in multiple cancer types and male infertility, highlighting its significance in understanding disease mechanisms. The study of HAUS1 in these disease models provides insights into potential therapeutic targets and prognostic biomarkers.
References:
1. Tang, Lei, Chen, Zhonghuo, Wei, Chao, Tang, Peng, Wang, Kai. 2024. The significance of HAUS1 and its relationship with immune microenvironment in hepatocellular carcinoma. In Journal of Cancer, 15, 1328-1341. doi:10.7150/jca.90298. https://pubmed.ncbi.nlm.nih.gov/38356703/
2. Liu, Shiwei, Yu, Yang, Xu, Jie, Wang, Yi, Li, Deng. 2024. Single-cell and bulk RNA-sequencing reveals mitosis-involved gene HAUS1 is a promising indicator for predicting prognosis and immune responses in prostate adenocarcinoma (PRAD). In Cell biology international, 48, 1169-1184. doi:10.1002/cbin.12191. https://pubmed.ncbi.nlm.nih.gov/38818762/
3. Yao, Qi, Ge, Xinqi, Lu, Zhichao, Shen, Jianhong, Chen, Jian. 2022. HAUS Augmin-Like Complex Subunit 1 Influences Tumour Microenvironment and Prognostic Outcomes in Glioma. In Journal of oncology, 2022, 8027686. doi:10.1155/2022/8027686. https://pubmed.ncbi.nlm.nih.gov/35865089/
4. Ustinova, Kseniya, Ruhnow, Felix, Gili, Maria, Surrey, Thomas. 2023. Microtubule binding of the human augmin complex is directly controlled by importins and Ran-GTP. In Journal of cell science, 136, . doi:10.1242/jcs.261096. https://pubmed.ncbi.nlm.nih.gov/37357828/
5. Cheung, Stephanie, Xie, Philip, Rosenwaks, Zev, Palermo, Gianpiero D. 2022. Profiling the male germline genome to unravel its reproductive potential. In Fertility and sterility, 119, 196-206. doi:10.1016/j.fertnstert.2022.11.006. https://pubmed.ncbi.nlm.nih.gov/36379263/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen