C57BL/6JCya-Gpsm1em1/Cya
Common Name:
Gpsm1-KO
Product ID:
S-KO-19129
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gpsm1-KO
Strain ID
KOCMP-67839-Gpsm1-B6J-VA
Gene Name
Product ID
S-KO-19129
Gene Alias
1810037C22Rik; Ags3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpsm1em1/Cya mice (Catalog S-KO-19129) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000066889
NCBI RefSeq
NM_001199147
Target Region
Exon 2~8
Size of Effective Region
~4.7 kb
Detailed Document
Overview of Gene Research
Gpsm1, also known as activator of G-protein signaling 3, is an accessory protein that modulates the G-protein signaling system. It is involved in multiple biological processes and associated with several pathways, such as those related to inflammation, metabolism, and cell-cycle regulation. Genetic models, especially KO/CKO mouse models, have been crucial for studying its functions [3].
In myeloid-specific Gpsm1-ablated mice, metabolic inflammation is repressed, protecting against high-fat diet-induced insulin resistance, glucose dysregulation, and liver steatosis. GPSM1 deficiency promotes TNFAIP3 transcription via the Gαi3/cAMP/PKA/CREB axis, inhibiting TLR4-induced NF-κB signaling in macrophages [1]. Mice lacking Gpsm1 in POMC neurons are protected against diet-induced obesity, glucose dysregulation, insulin resistance, and hepatic steatosis. Gpsm1 deficiency in these neurons enhances autophagy and leptin sensitivity through the PI3K/AKT/mTOR signaling, increasing POMC expression and α-MSH production, and enhancing sympathetic innervation and activity [2]. In an orthologous mouse model of autosomal dominant polycystic kidney disease, complete loss of Gpsm1 led to increased cyst progression and reduced renal function, and GPSM1 was shown to increase heteromeric polycystin-1/polycystin-2 ion channel activity via Gβγ subunits [3].
In conclusion, Gpsm1 plays essential roles in metabolic homeostasis, energy balance, and cyst progression in autosomal dominant polycystic kidney disease. The KO/CKO mouse models have significantly contributed to understanding its functions in these disease-related biological processes, providing potential therapeutic targets for metabolic disorders and polycystic kidney disease.
References:
1. Yan, Jing, Zhang, Yuemei, Yu, Hairong, Jia, Weiping, Hu, Cheng. 2022. GPSM1 impairs metabolic homeostasis by controlling a pro-inflammatory pathway in macrophages. In Nature communications, 13, 7260. doi:10.1038/s41467-022-34998-9. https://pubmed.ncbi.nlm.nih.gov/36434066/
2. Tang, Mengyang, Zhang, Yi, Zhang, Rong, Yan, Jing, Hu, Cheng. 2023. GPSM1 in POMC neurons impairs brown adipose tissue thermogenesis and provokes diet-induced obesity. In Molecular metabolism, 79, 101839. doi:10.1016/j.molmet.2023.101839. https://pubmed.ncbi.nlm.nih.gov/37979657/
3. Kwon, Michelle, Pavlov, Tengis S, Nozu, Kandai, Staruschenko, Alexander, Park, Frank. 2012. G-protein signaling modulator 1 deficiency accelerates cystic disease in an orthologous mouse model of autosomal dominant polycystic kidney disease. In Proceedings of the National Academy of Sciences of the United States of America, 109, 21462-7. doi:10.1073/pnas.1216830110. https://pubmed.ncbi.nlm.nih.gov/23236168/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen