C57BL/6JCya-Mcm8em1/Cya
Common Name
Mcm8-KO
Product ID
S-KO-19135
Backgroud
C57BL/6JCya
Strain ID
KOCMP-66634-Mcm8-B6J-VA
When using this mouse strain in a publication, please cite “Mcm8-KO Mouse (Catalog S-KO-19135) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Mcm8-KO
Strain ID
KOCMP-66634-Mcm8-B6J-VA
Gene Name
Product ID
S-KO-19135
Gene Alias
5730432L01Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000028831
NCBI RefSeq
NM_001291054
Target Region
Exon 11~12
Size of Effective Region
~1.9 kb
Overview of Gene Research
Mcm8, short for Minichromosome Maintenance 8 Homologous Recombination Repair Factor, is a key gene involved in multiple DNA-related processes. It is crucial for meiotic homologous recombination repair, safeguards genome stability during mitosis by coordinating the replication stress response, and is also implicated in DNA replication initiation [3]. Mcm8-related pathways are vital for maintaining genome integrity and play a role in processes like mitophagy and R-loop resolution [1,2]. Genetic models, such as mouse models, are valuable for studying its functions.
Disabling Mcm8 in mice leads to proliferation defects of primordial germ cells (PGCs), ultimately impairing fertility [1]. This shows its importance in PGC development and reproductive reserve establishment. Also, MCM8-deficient mice develop more severe coronary artery vasculopathy in a Kawasaki disease model, suggesting its role in protecting vascular health [2].
In conclusion, Mcm8 is essential for maintaining genome integrity, playing a significant role in processes like PGC development and vascular health. Mouse knockout models have been instrumental in revealing its functions in reproductive aging and Kawasaki disease-related vascular pathologies, enhancing our understanding of these biological processes and associated diseases.
References：
1. Wen, Canxin, Cao, Lili, Wang, Shuhan, Yang, Yajuan, Qin, Yingying. 2024. MCM8 interacts with DDX5 to promote R-loop resolution. In The EMBO journal, 43, 3044-3071. doi:10.1038/s44318-024-00134-0. https://pubmed.ncbi.nlm.nih.gov/38858601/
2. Lin, Meng, Xian, Huifang, Chen, Zhanghua, Gu, Xiaoqiong, Zhang, Yuxia. 2023. MCM8-mediated mitophagy protects vascular health in response to nitric oxide signaling in a mouse model of Kawasaki disease. In Nature cardiovascular research, 2, 778-792. doi:10.1038/s44161-023-00314-x. https://pubmed.ncbi.nlm.nih.gov/39195969/
3. Helderman, Noah Cornelis, Terlouw, Diantha, Bonjoch, Laia, Goldberg, Yael, Nielsen, Maartje. 2023. Molecular functions of MCM8 and MCM9 and their associated pathologies. In iScience, 26, 106737. doi:10.1016/j.isci.2023.106737. https://pubmed.ncbi.nlm.nih.gov/37378315/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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