C57BL/6JCya-Mcm8em1/Cya
Common Name:
Mcm8-KO
Product ID:
S-KO-19135
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mcm8-KO
Strain ID
KOCMP-66634-Mcm8-B6J-VA
Gene Name
Product ID
S-KO-19135
Gene Alias
5730432L01Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mcm8em1/Cya mice (Catalog S-KO-19135) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028831
NCBI RefSeq
NM_001291054
Target Region
Exon 11~12
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Mcm8, short for Minichromosome Maintenance 8 Homologous Recombination Repair Factor, is a key gene involved in multiple DNA-related processes. It is crucial for meiotic homologous recombination repair, safeguards genome stability during mitosis by coordinating the replication stress response, and is also implicated in DNA replication initiation [3]. Mcm8-related pathways are vital for maintaining genome integrity and play a role in processes like mitophagy and R-loop resolution [1,2]. Genetic models, such as mouse models, are valuable for studying its functions.
Disabling Mcm8 in mice leads to proliferation defects of primordial germ cells (PGCs), ultimately impairing fertility [1]. This shows its importance in PGC development and reproductive reserve establishment. Also, MCM8-deficient mice develop more severe coronary artery vasculopathy in a Kawasaki disease model, suggesting its role in protecting vascular health [2].
In conclusion, Mcm8 is essential for maintaining genome integrity, playing a significant role in processes like PGC development and vascular health. Mouse knockout models have been instrumental in revealing its functions in reproductive aging and Kawasaki disease-related vascular pathologies, enhancing our understanding of these biological processes and associated diseases.
References:
1. Wen, Canxin, Cao, Lili, Wang, Shuhan, Yang, Yajuan, Qin, Yingying. 2024. MCM8 interacts with DDX5 to promote R-loop resolution. In The EMBO journal, 43, 3044-3071. doi:10.1038/s44318-024-00134-0. https://pubmed.ncbi.nlm.nih.gov/38858601/
2. Lin, Meng, Xian, Huifang, Chen, Zhanghua, Gu, Xiaoqiong, Zhang, Yuxia. 2023. MCM8-mediated mitophagy protects vascular health in response to nitric oxide signaling in a mouse model of Kawasaki disease. In Nature cardiovascular research, 2, 778-792. doi:10.1038/s44161-023-00314-x. https://pubmed.ncbi.nlm.nih.gov/39195969/
3. Helderman, Noah Cornelis, Terlouw, Diantha, Bonjoch, Laia, Goldberg, Yael, Nielsen, Maartje. 2023. Molecular functions of MCM8 and MCM9 and their associated pathologies. In iScience, 26, 106737. doi:10.1016/j.isci.2023.106737. https://pubmed.ncbi.nlm.nih.gov/37378315/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen