C57BL/6JCya-Apol6em1/Cya
Common Name:
Apol6-KO
Product ID:
S-KO-19138
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Apol6-KO
Strain ID
KOCMP-71939-Apol6-B6J-VA
Gene Name
Product ID
S-KO-19138
Gene Alias
2310076O14Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Apol6em1/Cya mice (Catalog S-KO-19138) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000149569
NCBI RefSeq
NM_028010
Target Region
Exon 3
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Apol6, also known as Apolipoprotein L6, is a gene with diverse functions. In adipose tissue, it associates with lipid droplets and impacts adipocyte lipolysis by disrupting the Perilipin1-HSL interaction [1]. It is also involved in regulating the differentiation of intramuscular preadipocytes in goats [3]. In addition, Apol6 has potential connections to cancer-related processes, such as predicting immunotherapy efficacy in bladder cancer through ferroptosis [2], and being involved in radiosensitivity-related processes in rectal cancer [4].
In adipocytes, ApoL6 knockdown results in smaller lipid droplets with lower TAG content, while overexpression leads to larger lipid droplets with higher TAG content. ApoL6 ablation in mice decreases white adipose tissue mass, protecting them from diet-induced obesity, indicating its role in obesity-related mechanisms [1]. In bladder cancer, Apol6 was identified as a biomarker predicting the efficacy of immune checkpoint inhibitors, and it mediates immunotherapy efficacy by ferroptosis [2].
In conclusion, Apol6 plays crucial roles in lipid metabolism in adipose tissue, potentially contributing to obesity-related conditions. In the cancer context, it shows promise as a biomarker for predicting immunotherapy efficacy in bladder cancer and is associated with rectal cancer radiosensitivity. The study of Apol6 using gene-knockout models in mice has provided valuable insights into its functions in these biological processes and disease conditions.
References:
1. Wang, Yuhui, Nguyen, Hai P, Xue, Pengya, Duncan, Robin E, Sul, Hei S. 2024. ApoL6 associates with lipid droplets and disrupts Perilipin1-HSL interaction to inhibit lipolysis. In Nature communications, 15, 186. doi:10.1038/s41467-023-44559-3. https://pubmed.ncbi.nlm.nih.gov/38167864/
2. Fan, Zhiwei, Liu, Yiting, Wang, Xuehai, Zhao, Xinyuan, Liu, Lei. 2024. APOL6 predicts immunotherapy efficacy of bladder cancer by ferroptosis. In BMC cancer, 24, 1046. doi:10.1186/s12885-024-12820-7. https://pubmed.ncbi.nlm.nih.gov/39187773/
3. Qubi, Wuqie, Zheng, Jianying, Wang, Youli, Liu, Wei, Lin, Yaqiu. 2023. Goat miR-92a-3p Targets APOL6 Gene to Regulate the Differentiation of Intramuscular Precursor Adipocytes. In Genes, 15, . doi:10.3390/genes15010057. https://pubmed.ncbi.nlm.nih.gov/38254947/
4. Liu, Yi, Yang, Yanguang, Ni, Feng, Jiang, Xiaohui, Wang, Ding. 2023. Research on radiotherapy related genes and prognostic target identification of rectal cancer based on multi-omics. In Journal of translational medicine, 21, 856. doi:10.1186/s12967-023-04753-9. https://pubmed.ncbi.nlm.nih.gov/38012642/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen