C57BL/6JCya-Fam20bem1/Cya
Common Name:
Fam20b-KO
Product ID:
S-KO-19143
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fam20b-KO
Strain ID
KOCMP-215015-Fam20b-B6J-VA
Gene Name
Product ID
S-KO-19143
Gene Alias
C530043G21Rik; mKIAA0475
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fam20bem1/Cya mice (Catalog S-KO-19143) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000086153
NCBI RefSeq
NM_145413
Target Region
Exon 4
Size of Effective Region
~0.1 kb
Detailed Document
Overview of Gene Research
Fam20b, a member of the FAM20 kinase family, is a xylose kinase essential for glycosaminoglycan (GAG) assembly [3]. It phosphorylates the xylose residue in the glycosaminoglycan-protein linkage region, regulating the number of GAG chains [3]. GAGs are crucial components of the extracellular matrix (ECM), involved in various biological processes like signal molecule enrichment and ligand-receptor binding regulation [5].
In mouse models, inactivation of Fam20b in different tissues reveals its diverse roles. In dental epithelium, disruption of Fam20b-catalyzed GAGs leads to supernumerary tooth formation by affecting Sox2⁺ dental epithelial stem/progenitor cell homeostasis and restricting FGFR2b signaling [1,6]. In neural crest-derived mesenchyme, its inactivation causes multiple craniofacial defects including cleft palate, micrognathia, and reduced mineralization [2,5]. In type I collagen-expressing cells of the intervertebral disc annulus fibrosus, Fam20b inactivation results in disc defects and cell fate changes via alterations in TGF-β and MAPK signaling pathways [4].
In conclusion, Fam20b is vital for the proper development and function of multiple tissues through its role in GAG synthesis. The study of Fam20b knockout or conditional knockout mouse models has significantly contributed to understanding its functions in craniofacial development, tooth formation, and intervertebral disc development, providing insights into related disease mechanisms [1,2,4,5,6].
References:
1. Wu, Jingyi, Tian, Ye, Han, Lu, Linhardt, Robert J, Wang, Xiaofang. 2020. FAM20B-catalyzed glycosaminoglycans control murine tooth number by restricting FGFR2b signaling. In BMC biology, 18, 87. doi:10.1186/s12915-020-00813-4. https://pubmed.ncbi.nlm.nih.gov/32664967/
2. Chen, Xiaoyan, Li, Nan, Hu, Ping, Xiao, Jing, Liu, Chao. 2023. Deficiency of Fam20b-Catalyzed Glycosaminoglycan Chain Synthesis in Neural Crest Leads to Cleft Palate. In International journal of molecular sciences, 24, . doi:10.3390/ijms24119634. https://pubmed.ncbi.nlm.nih.gov/37298583/
3. Koike, Toshiyasu, Izumikawa, Tomomi, Tamura, Jun-Ichi, Kitagawa, Hiroshi. 2009. FAM20B is a kinase that phosphorylates xylose in the glycosaminoglycan-protein linkage region. In The Biochemical journal, 421, 157-62. doi:10.1042/BJ20090474. https://pubmed.ncbi.nlm.nih.gov/19473117/
4. Saiyin, Wuliji, Li, Lili, Zhang, Hua, Lu, Yongbo, Qin, Chunlin. 2019. Inactivation of FAM20B causes cell fate changes in annulus fibrosus of mouse intervertebral disc and disc defects via the alterations of TGF-β and MAPK signaling pathways. In Biochimica et biophysica acta. Molecular basis of disease, 1865, 165555. doi:10.1016/j.bbadis.2019.165555. https://pubmed.ncbi.nlm.nih.gov/31513834/
5. Liu, Xuena, Li, Nan, Zhang, Hua, Xiao, Jing, Liu, Chao. 2018. Inactivation of Fam20b in the neural crest-derived mesenchyme of mouse causes multiple craniofacial defects. In European journal of oral sciences, 126, 433-436. doi:10.1111/eos.12563. https://pubmed.ncbi.nlm.nih.gov/30105814/
6. Tian, Ye, Ma, Pan, Liu, Chao, Qin, Chunlin, Wang, Xiaofang. 2015. Inactivation of Fam20B in the dental epithelium of mice leads to supernumerary incisors. In European journal of oral sciences, 123, 396-402. doi:10.1111/eos.12222. https://pubmed.ncbi.nlm.nih.gov/26465965/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen