C57BL/6JCya-Cdkal1em1/Cya
Common Name:
Cdkal1-KO
Product ID:
S-KO-19152
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cdkal1-KO
Strain ID
KOCMP-68916-Cdkal1-B6J-VB
Gene Name
Product ID
S-KO-19152
Gene Alias
1190005B03Rik; 6620401C13Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cdkal1em1/Cya mice (Catalog S-KO-19152) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000006353
NCBI RefSeq
NM_144536
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Cdkal1, also known as CDK5 regulatory subunit Associated protein 1-Like 1, is a gene encoding a tRNA-modifying enzyme. It has an intrinsic thiomethyl transferase activity crucial for proper post-translational processing of pre-proinsulin to mature insulin. Cdkal1 is also an endogenous inhibitor of cdk5, preventing cdk5-induced interruption in insulin synthesis through PDX1 translocation [1]. It is involved in the pathophysiology of type 2 diabetes mellitus (T2DM) and its microvascular complications [1].
In both general and pancreatic β-cell-specific Cdkal1-deficient mice, impaired mitochondrial ATP generation and first-phase insulin secretion are observed, with β-cell-specific knockout mice showing pancreatic islet hypertrophy and impaired blood glucose control, and hypersensitivity to high-fat diet-induced ER stress [2]. Adipocyte-specific Cdkal1 deletion in mice leads to impaired mitochondrial function in brown adipocytes and BAT, decreased energy expenditure during cold challenge, and abnormal mitochondrial morphology in BAT. Novel protein interactors of CDKAL1, like SLC25A4/ANT1, are identified, and Cdkal1 A-KO mice have increased ANT1 protein levels in white adipose tissue [3].
In conclusion, Cdkal1 is essential for normal mitochondrial morphology and function in adipose tissue and proper insulin processing. Gene knockout mouse models, such as pancreatic β-cell-specific and adipocyte-specific Cdkal1-deficient mice, have revealed its role in T2DM pathogenesis, highlighting its importance in understanding the disease mechanism and potentially targeting it for T2DM management [1,2,3].
References:
1. Ghosh, Chandrachur, Das, Neeladrisingha, Saha, Sarama, Sircar, Debabrata, Roy, Partha. 2022. Involvement of Cdkal1 in the etiology of type 2 diabetes mellitus and microvascular diabetic complications: a review. In Journal of diabetes and metabolic disorders, 21, 991-1001. doi:10.1007/s40200-021-00953-6. https://pubmed.ncbi.nlm.nih.gov/35673487/
2. Wei, Fan-Yan, Tomizawa, Kazuhito. 2011. Functional loss of Cdkal1, a novel tRNA modification enzyme, causes the development of type 2 diabetes. In Endocrine journal, 58, 819-25. doi:. https://pubmed.ncbi.nlm.nih.gov/21908934/
3. Palmer, Colin J, Bruckner, Raphael J, Paulo, Joao A, Cohen, David E, Banks, Alexander S. 2017. Cdkal1, a type 2 diabetes susceptibility gene, regulates mitochondrial function in adipose tissue. In Molecular metabolism, 6, 1212-1225. doi:10.1016/j.molmet.2017.07.013. https://pubmed.ncbi.nlm.nih.gov/29031721/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen