C57BL/6JCya-Muc16em1/Cya
Common Name:
Muc16-KO
Product ID:
S-KO-19160
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Muc16-KO
Strain ID
KOCMP-73732-Muc16-B6J-VA
Gene Name
Product ID
S-KO-19160
Gene Alias
1110008I14Rik; Ca125; Gm1480; Gm21044
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Muc16em1/Cya mice (Catalog S-KO-19160) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000208663
NCBI RefSeq
XM_011242634
Target Region
Exon 1~78
Size of Effective Region
~179.7 kb
Detailed Document
Overview of Gene Research
Muc16, also known as carbohydrate antigen 125 (CA125), is a membrane-bound mucin expressed on normal body epithelium. It contains abundant glycosylation sites and participates in various molecular pathways [3,5]. Muc16 can interact with ligands like galectin-1/3, mesothelin, Siglec-9, and is involved in multiple signaling pathways such as mesenchymal protein, PI3K/AKT, JAK2/STAT3, ERK/FBW7/c-Myc, playing important roles in tumorigenesis, proliferation, migration, invasion, and tumor immunity [1,2].
Abnormal expression of Muc16 promotes tumor progression. For example, in triple-negative breast cancer, Muc16 promotes lung metastasis by modulating RNA-binding protein ELAVL1/HUR and the Muc16/HuR/cMyc axis [6]. In ovarian cancer, Muc16 acts on neutrophils through Siglec-9, leading to an inflammatory and immunosuppressive phenotype [4]. Also, Muc16 helps tumor immune escape by inhibiting T cells and NK cells [1].
In conclusion, Muc16 is a key molecule in normal and disease states, especially in tumors. Its abnormal expression significantly impacts tumor-related biological processes like progression and immune escape. Understanding Muc16 through functional studies provides insights into tumorigenesis mechanisms, which may help develop new strategies for cancer treatment [1,2,4,6].
References:
1. Zhang, Xin-Yu, Hong, Lian-Lian, Ling, Zhi-Qiang. 2024. MUC16: clinical targets with great potential. In Clinical and experimental medicine, 24, 101. doi:10.1007/s10238-024-01365-5. https://pubmed.ncbi.nlm.nih.gov/38758220/
2. Gao, Ruyun, Lou, Ning, Han, Xiaohong, Shi, Yuankai. . [MUC16: The Novel Target for Tumor Therapy]. In Zhongguo fei ai za zhi = Chinese journal of lung cancer, 25, 452-459. doi:10.3779/j.issn.1009-3419.2022.101.31. https://pubmed.ncbi.nlm.nih.gov/35899441/
3. Song, Yaan, Yuan, Ming, Wang, Guoyun. 2023. Update value and clinical application of MUC16 (cancer antigen 125). In Expert opinion on therapeutic targets, 27, 745-756. doi:10.1080/14728222.2023.2248376. https://pubmed.ncbi.nlm.nih.gov/37584221/
4. Wu, Yuliang, Liu, Qi, Xie, Yan, Liu, Shupeng, Cheng, Zhongping. 2023. MUC16 stimulates neutrophils to an inflammatory and immunosuppressive phenotype in ovarian cancer. In Journal of ovarian research, 16, 181. doi:10.1186/s13048-023-01207-0. https://pubmed.ncbi.nlm.nih.gov/37644468/
5. Haridas, Dhanya, Ponnusamy, Moorthy P, Chugh, Seema, Seshacharyulu, Parthasarathy, Batra, Surinder K. 2014. MUC16: molecular analysis and its functional implications in benign and malignant conditions. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 28, 4183-99. doi:10.1096/fj.14-257352. https://pubmed.ncbi.nlm.nih.gov/25002120/
6. Chaudhary, Sanjib, Appadurai, Muthamil Iniyan, Maurya, Shailendra Kumar, Batra, Surinder K, Lakshmanan, Imayavaramban. 2023. MUC16 promotes triple-negative breast cancer lung metastasis by modulating RNA-binding protein ELAVL1/HUR. In Breast cancer research : BCR, 25, 25. doi:10.1186/s13058-023-01630-7. https://pubmed.ncbi.nlm.nih.gov/36918912/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen