C57BL/6JCya-Hspd1em1/Cya
Common Name:
Hspd1-KO
Product ID:
S-KO-19168
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Hspd1-KO
Strain ID
KOCMP-15510-Hspd1-B6J-VA
Gene Name
Product ID
S-KO-19168
Gene Alias
60kDa; CPN60; HSP-60; HSP-65; Hsp60
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hspd1em1/Cya mice (Catalog S-KO-19168) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027123
NCBI RefSeq
NM_010477
Target Region
Exon 3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Hspd1, also known as mitochondrial heat shock protein 60 (HSP60), is a crucial mitochondrial chaperone. It functions as a folding machinery for mitochondrial proteins imported into the mitochondrial matrix space [3]. It is associated with stress response pathways and plays a role in maintaining mitochondrial integrity [2].
In osteosarcoma, Hspd1 expression is increased and correlates with negative prognosis. In vitro and in vivo experiments show it promotes osteosarcoma cell proliferation, metastasis, and inhibits apoptosis by promoting epithelial-mesenchymal transition (EMT) and activating AKT/mTOR signaling. It may achieve this by interacting with ATP5A1 to reduce its K48-linked ubiquitination and degradation [1].
In oral squamous cell carcinoma, higher Hspd1 protein levels are associated with tumorigenesis, poor prognosis, and lymph node invasion. Knockdown of Hspd1 induces E-cadherin expression and decreases cell migration and invasion, while its overexpression has the opposite effects [4].
In conclusion, Hspd1 is essential for mitochondrial protein folding and mitochondrial integrity. Its dysregulation is associated with various cancers, such as osteosarcoma and oral squamous cell carcinoma. Studies on Hspd1 help understand its role in cancer progression, providing potential therapeutic targets for these diseases.
References：
1. Zhang, Yiming, Pan, Ruilin, Li, Kun, He, Rongrong, Wang, Huajun. 2024. HSPD1 Supports Osteosarcoma Progression through Stabilizing ATP5A1 and thus Activation of AKT/mTOR Signaling. In International journal of biological sciences, 20, 5162-5190. doi:10.7150/ijbs.100015. https://pubmed.ncbi.nlm.nih.gov/39430254/
2. O'Malley, Jordan, Kumar, Rahul, Inigo, Joseph, Yadava, Nagendra, Chandra, Dhyan. 2020. Mitochondrial Stress Response and Cancer. In Trends in cancer, 6, 688-701. doi:10.1016/j.trecan.2020.04.009. https://pubmed.ncbi.nlm.nih.gov/32451306/
3. Cömert, Cagla, Brick, Lauren, Ang, Debbie, Fernandez-Guerra, Paula, Bross, Peter. 2020. A recurrent de novo HSPD1 variant is associated with hypomyelinating leukodystrophy. In Cold Spring Harbor molecular case studies, 6, . doi:10.1101/mcs.a004879. https://pubmed.ncbi.nlm.nih.gov/32532876/
4. Kang, Bor-Hwang, Shu, Chih-Wen, Chao, Jian-Kang, Ger, Luo-Ping, Liu, Pei-Feng. 2019. HSPD1 repressed E-cadherin expression to promote cell invasion and migration for poor prognosis in oral squamous cell carcinoma. In Scientific reports, 9, 8932. doi:10.1038/s41598-019-45489-1. https://pubmed.ncbi.nlm.nih.gov/31222140/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen