C57BL/6JCya-Srpraem1/Cya
Common Name:
Srpra-KO
Product ID:
S-KO-19181
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Srpra-KO
Strain ID
KOCMP-67398-Srpra-B6J-VB
Gene Name
Product ID
S-KO-19181
Gene Alias
1300011P19Rik; D11Mgi27; Srpr
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Srpraem1/Cya mice (Catalog S-KO-19181) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034541
NCBI RefSeq
NM_026130
Target Region
Exon 4~14
Size of Effective Region
~3.4 kb
Detailed Document
Overview of Gene Research
Srpra, the signal recognition particle receptor alpha, is a key constituent of the mammalian cotranslational targeting machinery. It is involved in protein processing, intracellular trafficking, and homeostasis, which are crucial for the differentiation of neutrophil granulocytes [1]. It may also be associated with pathways like apoptosis, chaperon-related processes, unfolded protein response, cell adhesion, and cellular stress [2].
In a study on severe congenital neutropenia, genetic defects in SRPRA were discovered, leading to distinctive proteome changes in neutrophil granulocytes. By using in vitro differentiation of human induced pluripotent stem cells and in vivo zebrafish models, the effects of SRP deficiency (including that related to Srpra) on neutrophil granulocyte development were studied, highlighting the importance of Srpra-dependent processes in this cell differentiation [1].
In a study on keratoconus, genes including Srpra, whose expression in the corneal epithelium was correlated with eye-rubbing manner, were enriched in several key pathways, suggesting Srpra's potential role in the cellular response to mechanical corneal trauma [2].
In a study on bronchopulmonary dysplasia, Srpra was identified as a key hub gene, with related genes involved in pathways such as Golgi vesicle transport, which may be associated with the pathological process of BPD [3].
In conclusion, Srpra plays essential roles in protein-related cellular processes, especially in neutrophil granulocyte differentiation. Studies using various models have also implicated its role in diseases like severe congenital neutropenia, keratoconus, and bronchopulmonary dysplasia. These model-based research findings help us understand the biological functions of Srpra and its potential significance in disease mechanisms.
References:
1. Linder, Monika I, Mizoguchi, Yoko, Hesse, Sebastian, Lieschke, Graham J, Klein, Christoph. . Human genetic defects in SRP19 and SRPRA cause severe congenital neutropenia with distinctive proteome changes. In Blood, 141, 645-658. doi:10.1182/blood.2022016783. https://pubmed.ncbi.nlm.nih.gov/36223592/
2. Jaskiewicz, Katarzyna, Maleszka-Kurpiel, Magdalena, Michalski, Andrzej, Rydzanicz, Malgorzata, Gajecka, Marzena. 2023. Non-allergic eye rubbing is a major behavioral risk factor for keratoconus. In PloS one, 18, e0284454. doi:10.1371/journal.pone.0284454. https://pubmed.ncbi.nlm.nih.gov/37053215/
3. Yu, Xuefei, Liu, Ziyun, Pan, Yuqing, Xue, Xindong, Fu, Jianhua. 2022. Co-expression network analysis for identification of novel biomarkers of bronchopulmonary dysplasia model. In Frontiers in pediatrics, 10, 946747. doi:10.3389/fped.2022.946747. https://pubmed.ncbi.nlm.nih.gov/36440350/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen