C57BL/6JCya-Ddx54em1/Cya
Common Name:
Ddx54-KO
Product ID:
S-KO-19213
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ddx54-KO
Strain ID
KOCMP-71990-Ddx54-B6J-VA
Gene Name
Product ID
S-KO-19213
Gene Alias
2410015A15Rik; DP97
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ddx54em1/Cya mice (Catalog S-KO-19213) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031598
NCBI RefSeq
NM_028041
Target Region
Exon 3~10
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Ddx54, also known as Dbp10, is a DEAD-box RNA helicase. It plays essential roles in various biological processes. It is involved in ribosome biogenesis, specifically initiating peptidyl transferase center formation during 60S ribosome biogenesis [4,5]. It also functions as a nucleotide exchange factor for casein kinase 2, stimulating its protein kinase activity [6].
In disease-related studies, in colorectal cancer, Ddx54 is overexpressed. High levels are correlated with tumor stage and distant metastasis, and it promotes CRC cell proliferation and mobility by activating p65 and AKT signaling pathways [1]. In gastric cancer, Ddx54 binds to SNHG10 and PBX3, and SNHG10 maintains PBX3 mRNA stability through recruiting Ddx54, facilitating cell growth [3]. In osteosarcoma, lncRNA CBR3-AS1 recruits Ddx54 to upregulate NUCKS1, activating the mTOR signaling pathway and promoting tumor progression [7]. In multiple sclerosis, oligodendrocyte-specific Ddx54 knockout mice gradually develop myelin-related abnormalities, indicating Ddx54 is indispensable for myelin homeostasis [2].
In summary, Ddx54 is crucial for ribosome biogenesis and acts as a nucleotide exchange factor. Its dysregulation is associated with multiple cancers and a demyelinating disease. Studies using gene knockout models, especially in mice, have revealed its role in these biological processes and disease conditions, providing insights into potential therapeutic strategies.
References:
1. Yu, Yi, Wang, Jing-Long, Meng, Li-Li, Fu, Guo-Hui, Zu, Li-Dong. 2021. DDX54 Plays a Cancerous Role Through Activating P65 and AKT Signaling Pathway in Colorectal Cancer. In Frontiers in oncology, 11, 650360. doi:10.3389/fonc.2021.650360. https://pubmed.ncbi.nlm.nih.gov/33968751/
2. Oizumi, Hiroaki, Miyamoto, Yuki, Seiwa, Chika, Yamauchi, Junji, Asou, Hiroaki. 2023. Lethal adulthood myelin breakdown by oligodendrocyte-specific Ddx54 knockout. In iScience, 26, 107448. doi:10.1016/j.isci.2023.107448. https://pubmed.ncbi.nlm.nih.gov/37720086/
3. Zhang, Yunfei, Guo, Hongyan, Zhang, Hong. 2020. SNHG10/DDX54/PBX3 Feedback Loop Contributes to Gastric Cancer Cell Growth. In Digestive diseases and sciences, 66, 1875-1884. doi:10.1007/s10620-020-06488-9. https://pubmed.ncbi.nlm.nih.gov/32712782/
4. Cruz, Victor E, Weirich, Christine S, Peddada, Nagesh, Erzberger, Jan P. 2024. The DEAD-box ATPase Dbp10/DDX54 initiates peptidyl transferase center formation during 60S ribosome biogenesis. In Nature communications, 15, 3296. doi:10.1038/s41467-024-47616-7. https://pubmed.ncbi.nlm.nih.gov/38632236/
5. Cruz, Victor E, Weirich, Christine S, Peddada, Nagesh, Erzberger, Jan P. 2023. The DEAD-box ATPase Dbp10/DDX54 initiates peptidyl transferase center formation during 60S ribosome biogenesis. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.11.01.565222. https://pubmed.ncbi.nlm.nih.gov/37961218/
6. Fatti, Edoardo, Hirth, Alexander, Švorinić, Andrea, Höfer, Thomas, Niehrs, Christof. 2023. DEAD box RNA helicases act as nucleotide exchange factors for casein kinase 2. In Science signaling, 16, eabp8923. doi:10.1126/scisignal.abp8923. https://pubmed.ncbi.nlm.nih.gov/37098120/
7. Yao, Weitao, Hou, Jingyu, Liu, Guoqing, Guo, Liangyu, Wang, Chuchu. 2022. LncRNA CBR3-AS1 promotes osteosarcoma progression through the network of miR-140-5p/DDX54-NUCKS1-mTOR signaling pathway. In Molecular therapy oncolytics, 25, 189-200. doi:10.1016/j.omto.2022.03.001. https://pubmed.ncbi.nlm.nih.gov/35592388/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen