C57BL/6JCya-Gdf11em1/Cya
Common Name:
Gdf11-KO
Product ID:
S-KO-19217
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Gdf11-KO
Strain ID
KOCMP-14561-Gdf11-B6J-VB
Gene Name
Product ID
S-KO-19217
Gene Alias
BMP-11; Bmp11
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gdf11em1/Cya mice (Catalog S-KO-19217) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026408
NCBI RefSeq
NM_010272
Target Region
Exon 3
Size of Effective Region
~4.0 kb
Detailed Document
Overview of Gene Research
Gdf11, also known as bone morphogenetic protein 11 (BMP11), is a member of the Transforming Growth Factor β superfamily. It plays crucial roles in embryogenesis, cell cycle control, apoptosis, and cell differentiation. Gdf11 is involved in lipid metabolism, inflammatory processes, and aging, and is strongly related to carcinogenesis [1,5,7,8].
In the brain, selective knockout of Gdf11 in post-mitotic excitatory neurons in mice induces brain-aging-related transcriptional changes, neuronal senescence, hyperexcitability, dendritic pruning, synaptic input impairment, memory deficits, and shortened lifespan, indicating that endogenous Gdf11 acts as a brake on excitatory neuronal senescence and brain aging [2]. Systemic administration of Gdf11 in aged mice improves memory, alleviates senescence and depression-like symptoms through enhancing neuronal autophagy, and analysis of sera from young adults with major depressive disorder shows reduced Gdf11 levels [3]. In a murine model of corticosterone-induced depression-like phenotype, Gdf11 attenuates depressive-like behavior. In diabetic mice, topical application of Gdf11 promotes wound healing by stimulating endothelial progenitor cell mobilization and neovascularization mediated by the HIF-1ɑ-VEGF/SDF-1ɑ pathway [4]. In osteoarthritis, Gdf11 conditional knockout mice show NLRP3 inflammasome activation, inflammation, and metabolic dysfunction, while intra-articular administration of recombinant human Gdf11 alleviates OA pathogenesis, suggesting Gdf11 can suppress TNF-α-induced inflammation and cartilage degeneration by inhibiting NLRP3 inflammasome activation [6].
In conclusion, Gdf11 is a key regulator in multiple biological processes. Gene knockout and conditional knockout mouse models have revealed its important roles in brain aging, depression, diabetic wound healing, and osteoarthritis. These findings suggest Gdf11 could be a potential therapeutic target for related diseases.
References:
1. Król, Wojciech, Machelak, Weronika, Zielińska, Marta. 2023. GDF11 as a friend or an enemy in the cancer biology? In Biochimica et biophysica acta. Reviews on cancer, 1878, 188944. doi:10.1016/j.bbcan.2023.188944. https://pubmed.ncbi.nlm.nih.gov/37356738/
2. Wang, Di-Xian, Dong, Zhao-Jun, Deng, Sui-Xin, Shu, Yousheng, Zhao, Jing-Wei. 2023. GDF11 slows excitatory neuronal senescence and brain ageing by repressing p21. In Nature communications, 14, 7476. doi:10.1038/s41467-023-43292-1. https://pubmed.ncbi.nlm.nih.gov/37978295/
3. Moigneu, Carine, Abdellaoui, Soumia, Ramos-Brossier, Mariana, Lledo, Pierre-Marie, Katsimpardi, Lida. 2023. Systemic GDF11 attenuates depression-like phenotype in aged mice via stimulation of neuronal autophagy. In Nature aging, 3, 213-228. doi:10.1038/s43587-022-00352-3. https://pubmed.ncbi.nlm.nih.gov/37118117/
4. Zhang, Ying, Zhang, Yi-Yuan, Pan, Zhen-Wei, Jiao, Lei, Yang, Bao-Feng. 2022. GDF11 promotes wound healing in diabetic mice via stimulating HIF-1ɑ-VEGF/SDF-1ɑ-mediated endothelial progenitor cell mobilization and neovascularization. In Acta pharmacologica Sinica, 44, 999-1013. doi:10.1038/s41401-022-01013-2. https://pubmed.ncbi.nlm.nih.gov/36347996/
5. Habibi, Pardis, Falamarzi, Kimia, Ebrahimi, Niloofar Dehdari, Malekpour, Mahdi, Azarpira, Negar. . GDF11: An emerging therapeutic target for liver diseases and fibrosis. In Journal of cellular and molecular medicine, 28, e18140. doi:10.1111/jcmm.18140. https://pubmed.ncbi.nlm.nih.gov/38494851/
6. Zhang, Pengfei, Zhai, Haoxin, Zhang, Shuai, Zhao, Yunpeng, Zhang, Lei. 2024. GDF11 protects against mitochondrial-dysfunction-dependent NLRP3 inflammasome activation to attenuate osteoarthritis. In Journal of advanced research, , . doi:10.1016/j.jare.2024.08.001. https://pubmed.ncbi.nlm.nih.gov/39103049/
7. Simoni-Nieves, Arturo, Gerardo-Ramírez, Monserrat, Pedraza-Vázquez, Gibrán, Gomez-Quiroz, Luis E, Gutiérrez-Ruiz, María Concepción. 2019. GDF11 Implications in Cancer Biology and Metabolism. Facts and Controversies. In Frontiers in oncology, 9, 1039. doi:10.3389/fonc.2019.01039. https://pubmed.ncbi.nlm.nih.gov/31681577/
8. Machelak, Weronika, Szczepaniak, Adrian, Jacenik, Damian, Zielińska, Marta. 2023. The role of GDF11 during inflammation - An overview. In Life sciences, 322, 121650. doi:10.1016/j.lfs.2023.121650. https://pubmed.ncbi.nlm.nih.gov/37011872/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen